Laboratory experiments with growth potential of Cenangium ferruginosum tested on natural nutrition soils

Abstract

Abstract Začiatkom jari 2012 sa v niektorych oblastiach Slovenska objavilo važne poškodenie borovic. Hnednutie ihlic všetkych ročnikov, nekrozy kory a rakovinove rany na vetvach boli priznakmi tohto poškodenia. Neboli zistene poškodenia podkornym a listožravym hmyzom, koreňove hniloby, sypavky a ani tracheomykozy. Taktiež sme vylučili pyknidovku beľovu Sphaeropsis sapinea ako povodcu tohto hynutia borovic, hoci poškodenie borovic touto hubou bolo v obdobi rokov 2000 až 2007 najvyznamnejšie a v menšom rozsahu pretrvava stale. Laboratornymi rozbormi sme zistili pritomnosť huby Cenangium ferruginosum, ziskali sme čiste kultury a infekčnymi testami sme overili patogenitu ziskaneho izolatu. Potencialnu patogenitu sme testovali ako schopnosť mycelia rasť na prirodzenych živnych podach pripravenych z vyluhu listov, resp. dreva a kory vetvičiek vybranych drevin. Poznatky z terennych šetreni, laboratornych rozborov a terennych hodnoteni možnych predispozičnych faktorov su opisane v tomto članku. Abstract Serious pine dieback was reported in early spring from several localities in Slovakia in 2012. Needle necrosis, bark necrosis and twig cankers were the most conspicuous symptoms on diseased trees. There were no or at least not significant damages caused by bark beetles, leaf eating insects, root rots neither tracheomycosis. We also excluded Sphaeropsis sapinea (Fr.) Dyko & B. Sutton as the main pest agent, which played an important role in Pinus nigra Arnold dieback from 2000 to 2007 in Slovakia. Our laboratory inspections revealed Cenangium ferruginosum Fr. as the agent responsible for that dieback. We tested its growth capability on different natural nutrition soils in the laboratory to see the potential pathogenecity. This paper describes the pine dieback based on the field inspections and laboratory studies, and we discuss the role of predisposing factors involved in the dieback.