Investigation of Antioxidant and Cytotoxic Activities of Opuntia ficus-indica (L.) Mill. Fruit extract

Abstract

DOI : 10.26650/IstanbulJPharm.2019.19035 Opuntia ficus-indica fruits are claimed to possess several health-beneficial properties. This study was carried out to determine the phytochemicals as well as to compare antioxidant activity and cell viability of the methanol extract of OFI-fruits. Antioxidant activity was evaluated by various in vitro tests such as DPPH, ABTS. Furthermore LC-MS and HPLC analysis were performed. The activity of the OFI-fruit extract on viability of U87-MG (glioblastoma multiform), HT-29 (colon cancer) and human primary fibroblast cells has been investigated by determining viable cells on a luciferase based system (Cell TiterGlo; Promega;USA). The results showed that OFI-fruits were source of antioxidant compounds. Moreover, the extract was found to have rich phenolic-flavonoid contents. Quercetin was found in the extract according to LC-MS and HPLC studies. The remarkable increased cell death in the HT-29 and U87 cell lines was detected in a concentration-dependent manner (1-10mg/ml). Among these cell lines, HT-29 responded to OFI-fruit treatment more sensitively to even the lowest dose (1mg/ml). The results of this study showed the OFI-fruit has significant antioxidant capacity and leads to strong cytotoxic activity on various cancer cells. Therefore, it can be suggested that OFI-fruits may have chemo-preventive potential and can be studied further to clarify cytotoxic abilities. You may cite this article as : Okur ME, Karakas N, Karadag AE, Uludag D, Polat DC (2019). Investigation of Antioxidant and Cytotoxic Activities of Opuntia ficus-indica (L.) Mill. Fruit extract. Istanbul J Pharm 10.26650/IstanbulJPharm.2019.19035.