{"title":"STUDY OF STRUCTURAL TRANSITIONS OF COMPLEXES OF DIFFERENT LIGANDS WITH DNA AND BSA","authors":"P. Vardevanyan, Nara R. Petrosyan","doi":"10.46991/pysu:b/2022.56.1.049","DOIUrl":null,"url":null,"abstract":"Structural transitions of DNA and bovine serum albumin (BSA) and their complexes with methylene blue (MB) and Hoechst 33258 (H33258) by UV-denaturation method have been studied. The obtained results revel that MB stabilizes both protein dimensional structure and DNA double-stranded structure, while H33258 stabilizes double-stranded structure of DNA and destabilizes protein structure. It was also revealed that the stabilizing effect of MB on native structure of DNA is more pronounced at relatively low ligand concentrations, than on protein.","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":"37 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the YSU B: Chemical and Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.46991/pysu:b/2022.56.1.049","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Structural transitions of DNA and bovine serum albumin (BSA) and their complexes with methylene blue (MB) and Hoechst 33258 (H33258) by UV-denaturation method have been studied. The obtained results revel that MB stabilizes both protein dimensional structure and DNA double-stranded structure, while H33258 stabilizes double-stranded structure of DNA and destabilizes protein structure. It was also revealed that the stabilizing effect of MB on native structure of DNA is more pronounced at relatively low ligand concentrations, than on protein.
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