Characterisation of a potyvirus from Centrosema pubescens Benth.

Tropical Science Pub Date : 2007-03-01 DOI:10.1002/TS.192

O. Odedara, J. Hughes, S. Tarawali, A. Odebode, S. Winter

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引用次数: 2

Abstract

A virus, 750 × 18 nm, was isolated from Centrosema pubescens from Nigeria, causing leaf malformation, mosaic and mottling on the plant. When inoculated on to various test plants, it failed to produce necrotic or chlorotic lesions on Chenopodium amaranticolor, but symptoms of mottling were observed. The virus did not infect cowpea (Vigna unguiculata varieties IT82E-10, IT84S-2114, Ife brown, TVU 76 or TVU 2657), but produced chlorotic spots, vein clearing and mottling on soybean (Glycine max cultivars Malayan and Samsoy-2). Protein-A sandwich enzyme-linked immunosorbent assay (ELISA) shows that the virus is serologically closely related to Sweet potato feathery mottle virus (SPFMV) genus Potyvirus, Bean yellow mosaic virus (BYMV) genus Potyvirus and Tobacco etch virus (TEV) genus Potyvirus. Comparison of amino acid sequences of the coat protein of the virus with several potyvirus coat protein sequences, using BLAST (Basic Local Alignment Search Tool) analysis, confirmed the relationships. The antiserum raised against the virus had a titre value greater than 1 : 16,000, using antigen-coated plate ELISA. The name Centrosema mosaic virus (CenMoV) for this apparently new Potyvirus is suggested. The consequences of infection of forage legumes with this virus are discussed. Copyright © 2007 John Wiley & Sons, Ltd

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一株丛状芽孢中心病毒的鉴定。

摘要从尼日利亚毛蕊花(Centrosema pubescens)中分离到一株病毒，病毒长度为750 × 18 nm，可引起毛蕊花叶片畸形、花叶和斑驳。在不同的试验植株上接种后，均未在苋菜上产生坏死或褪绿的损伤，但出现了斑驳的症状。该病毒不侵染豇豆(Vigna unguiculata品种IT82E-10、IT84S-2114、Ife brown、TVU 76和TVU 2657)，但侵染大豆(Glycine max品种Malayan和Samsoy-2)后产生绿斑、脉清和斑纹。蛋白- a夹心酶联免疫吸附试验(ELISA)结果表明，该病毒与甘薯羽毛斑驳病毒(SPFMV)属Potyvirus、大豆黄花叶病毒(BYMV)属Potyvirus和烟草刻花病毒(TEV)属Potyvirus有血清学亲缘关系。利用BLAST (Basic Local Alignment Search Tool)分析方法，将该病毒外壳蛋白的氨基酸序列与几种痘病毒外壳蛋白序列进行比较，证实了它们之间的关系。采用抗原包被板酶联免疫吸附试验，获得的抗病毒血清滴度大于1:16 000。建议将这一明显的新型Potyvirus命名为centerrosema mosaic virus (CenMoV)。讨论了饲料豆科植物感染该病毒的后果。版权所有©2007 John Wiley & Sons, Ltd

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Tropical Science

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