Optimization of Secure Workstation for manipulation of 35S-labelled molecules: a case of in vitro cell metabolic labelling

Abstract

Pulse-chase or cell metabolic experiments involving 35S labelled molecules are deemed to be volatile. Neither the suppliers nor the literature propose any solutions to limit contamination. The aim of this study is to fill in this gap. Here, we expose the usual procedures, which consist in working with the available equipment. As this procedure leads to internal and external contaminations of the operators, we tested a more secure workstation with collective protection equipment that we validated as limiting contaminations for workers. This study proposes a method to use 35S labelled molecules in a more secure way, which could be applied in any laboratory.