Biophysical characterisation of hydrophobin enriched foamate

Cerevisia Pub Date : 2014-01-01 DOI:10.1016/j.cervis.2014.04.003

Mohammadreza Khalesi , Nathalie Mandelings , Zahra Shokribousjein , David Riveros-Galan , Hubert Verachtert , Kurt Gebruers , Frank Delvigne , Ivo Vankelecom , Guy Derdelinckx

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引用次数: 14

Abstract

Food industries are interested in finding highly stable foaming agents for product texture improvements. Hydrophobins have been mentioned many times in that context. For the production and purification of hydrophobin HFBII from Trichoderma reesei we found that foam fractionation of the fungal fermentation media is an interesting first step. After this step, the biophysical properties of the obtained foamate were studied. The foam generated by CO₂ as the only sparging gas into a HFBII enriched solution in a closed environment was more stable than the foam generated by sparging with air. The equilibrium surface tension of a foamate containing 100 mg/L HFBII was measured by CAMTEL CDCA-100 tensiometry as equal to 40 mJ/m², and was confirmed by the maximum bubble pressure tensiometer. Besides, it was found that for a long term foam preservation, the foam created by the HFBII-SDS combination is more stable than that of SDS.

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富疏水蛋白甲酸酯的生物物理特性

食品工业对寻找高度稳定的发泡剂以改善产品质地感兴趣。在这种情况下，疏水性化合物已经被提到了很多次。为了从里氏木霉中生产和纯化疏水蛋白HFBII，我们发现真菌发酵培养基的泡沫分馏是一个有趣的第一步。在此步骤之后，对所得的甲酸酯的生物物理性质进行了研究。封闭环境下，CO2作为唯一的喷吹气体进入HFBII富集溶液产生的泡沫比空气喷吹产生的泡沫更稳定。CAMTEL CDCA-100张力仪测得含100 mg/L HFBII的甲酸酯的平衡表面张力为40 mJ/m2，并通过最大气泡压力张力仪确认。此外，对于长期泡沫保存，HFBII-SDS组合产生的泡沫比SDS更稳定。

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