H2-induced transient upregulation of phospholipids with suppression of energy metabolism.

IF 3 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Medical Gas Research Pub Date : 2023-07-01 DOI:10.4103/2045-9912.344973
Masumi Iketani, Iwao Sakane, Yasunori Fujita, Masafumi Ito, Ikuroh Ohsawa
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引用次数: 1

Abstract

Molecular hydrogen (H2) is an antioxidant and anti-inflammatory agent; however, the molecular mechanisms underlying its biological effects are largely unknown. Similar to other gaseous molecules such as inhalation anesthetics, H2 is more soluble in lipids than in water. A recent study demonstrated that H2 reduces radical polymerization-induced cellular damage by suppressing fatty acid peroxidation and membrane permeability. Thus, we sought to examine the effects of short exposure to H2 on lipid composition and associated physiological changes in SH-SY5Y neuroblastoma cells. We analyzed cells by liquid chromatography-high-resolution mass spectrometry to define changes in lipid components. Lipid class analysis of cells exposed to H2 for 1 hour revealed transient increases in glycerophospholipids including phosphatidylethanolamine, phosphatidylinositol, and cardiolipin. Metabolomic analysis also showed that H2 exposure for 1 hour transiently suppressed overall energy metabolism accompanied by a decrease in glutathione. We further observed alterations to endosomal morphology by staining with specific antibodies. Endosomal transport of cholera toxin B to recycling endosomes localized around the Golgi body was delayed in H2-exposed cells. We speculate that H2-induced modification of lipid composition depresses energy production and endosomal transport concomitant with enhancement of oxidative stress, which transiently stimulates stress response pathways to protect cells.

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h2诱导的瞬时磷脂上调与能量代谢的抑制。
分子氢(H2)是一种抗氧化剂和抗炎剂;然而,其生物学作用的分子机制在很大程度上是未知的。与其他气体分子(如吸入性麻醉剂)类似,H2在脂质中比在水中更易溶解。最近的一项研究表明,H2通过抑制脂肪酸过氧化和膜通透性来减少自由基聚合引起的细胞损伤。因此,我们试图研究短时间暴露于H2对SH-SY5Y神经母细胞瘤细胞脂质组成和相关生理变化的影响。我们通过液相色谱-高分辨率质谱分析细胞来确定脂质成分的变化。对暴露于H2 1小时的细胞进行脂质分类分析,发现甘油磷脂(包括磷脂酰乙醇胺、磷脂酰肌醇和心磷脂)瞬间升高。代谢组学分析还表明,H2暴露1小时会短暂抑制总能量代谢,并伴有谷胱甘肽的减少。我们通过特异性抗体染色进一步观察到内体形态的改变。在h2暴露的细胞中,霍乱毒素B向高尔基体周围的再循环内体的内体运输被延迟。我们推测,h2诱导的脂质成分修饰抑制了能量产生和内体运输,同时增强了氧化应激,从而短暂地刺激应激反应途径以保护细胞。
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来源期刊
Medical Gas Research
Medical Gas Research MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
5.10
自引率
13.80%
发文量
35
期刊介绍: Medical Gas Research is an open access journal which publishes basic, translational, and clinical research focusing on the neurobiology as well as multidisciplinary aspects of medical gas research and their applications to related disorders. The journal covers all areas of medical gas research, but also has several special sections. Authors can submit directly to these sections, whose peer-review process is overseen by our distinguished Section Editors: Inert gases - Edited by Xuejun Sun and Mark Coburn, Gasotransmitters - Edited by Atsunori Nakao and John Calvert, Oxygen and diving medicine - Edited by Daniel Rossignol and Ke Jian Liu, Anesthetic gases - Edited by Richard Applegate and Zhongcong Xie, Medical gas in other fields of biology - Edited by John Zhang. Medical gas is a large family including oxygen, hydrogen, carbon monoxide, carbon dioxide, nitrogen, xenon, hydrogen sulfide, nitrous oxide, carbon disulfide, argon, helium and other noble gases. These medical gases are used in multiple fields of clinical practice and basic science research including anesthesiology, hyperbaric oxygen medicine, diving medicine, internal medicine, emergency medicine, surgery, and many basic sciences disciplines such as physiology, pharmacology, biochemistry, microbiology and neurosciences. Due to the unique nature of medical gas practice, Medical Gas Research will serve as an information platform for educational and technological advances in the field of medical gas.
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