The roles of BST-2 in murine B cell development and on virus propagation

IF 1.9 4区医学 Q4 IMMUNOLOGY Microbiology and Immunology Pub Date : 2023-01-05 DOI:10.1111/1348-0421.13049

Shuzo Urata, Sachiko Yamaguchi, Aya Nambu, Katsuko Sudo, Susumu Nakae, Jiro Yasuda

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Abstract

The bone marrow (BM) stromal cell antigen-2 (BST-2), also known as tetherin, CD317, PDCA-1, or HM1.24, is a membrane protein overexpressed in several types of tumors and may act as a promising target for cancer treatment via antibody-dependent cellular cytotoxicity. BST-2 is also expressed in human BM stromal cells (BMSC), which support B cell development. While the activity of BST-2 as an antiviral factor has been demonstrated, the expression patterns and the role of BST-2 on B-cell development and activation have not been investigated, especially in vivo. In this study, Bst2 knockout (Bst2^−/−) mice were generated to assess the role of BST-2 on B cell development and activation. It was observed that BST-2 was not expressed in BMSC or all B cell progenitors even in wild-type mice and does not play a significant role in B cell development. In addition, the loss of BST-2 had no effect on B cell activation. Furthermore and in contrast to the well-known antiviral role of BST-2, infection of vesicular stomatitis Indiana virus to the BM cells collected from the Bst2^−/− mice produced less infectious virus compared with that from the WT mice. These results suggest that murine BST-2 is different from human BST-2 in the expression pattern, physiological function, in vivo, and might possess positive role on VSV replication.

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BST-2在小鼠B细胞发育和病毒繁殖中的作用

骨髓(BM)基质细胞抗原-2 (BST-2)，也被称为tetherin、CD317、PDCA-1或HM1.24，是一种在几种类型肿瘤中过表达的膜蛋白，可能通过抗体依赖性细胞毒性作为癌症治疗的有希望的靶点。BST-2也在支持B细胞发育的人骨髓基质细胞(BMSC)中表达。虽然BST-2作为抗病毒因子的活性已被证实，但BST-2的表达模式及其在b细胞发育和激活中的作用尚未被研究，特别是在体内。本研究通过Bst2敲除(Bst2−/−)小鼠来评估BST-2在B细胞发育和激活中的作用。结果表明，即使在野生型小鼠中，BST-2也不表达于BMSC或所有B细胞祖细胞中，并且在B细胞发育中没有显著作用。此外，BST-2的缺失对B细胞的活化没有影响。此外，与BST-2众所周知的抗病毒作用相反，从BST-2−/−小鼠收集的BM细胞感染水疱性口炎印第安纳病毒产生的感染性病毒比从WT小鼠收集的病毒少。上述结果提示，小鼠BST-2在体内表达模式、生理功能等方面与人BST-2存在差异，可能对VSV的复制具有积极作用。

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.