PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri

Revista de la Facultad de Ciencias Agrarias UNCuyo Pub Date : 2023-07-05 DOI:10.48162/rev.39.101

Ana Paula Maia dos Santos, E. Santos, Juliana Silva de Oliveira, Gherman Garcia Leal de Araújo, Anderson de Moura Zanine, Ricardo Martins Araújo Pinho, Thiago Vinícius Costa do Nascimento, Alexandre Fernandes Perazzo, D. Ferreira, Alberto J. da Silva Macêdo, F. N. de Sousa Santos

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Abstract

This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 105 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage. Highlights: Lactic acid bacteria showed greater development at 7 days of fermentation. Lactobacillus plantarum predominated at 70 days, representing 93% of the total LAB population. Lactobacillus buchneri improved its microbiological profile with decreased ethanol production.

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冻干或活化布氏乳杆菌接种玉米青贮乳酸菌群体的PCR鉴定

本研究旨在评价接种冻干和/或活化的布氏乳杆菌对玉米青贮乳酸菌种群的影响。试验处理包括不添加添加剂的玉米青贮或青贮与布氏乳杆菌接种剂(1 × 105 cfu/g)，根据制造商的建议(1 g/吨饲料)以冻干接种剂和预活化接种剂的形式施用。从添加和未添加接种剂的玉米青贮中分离得到的纯化菌株中，93%为植物乳杆菌属乳酸菌。分离菌中未检出布氏乳杆菌。冻干或活化布氏乳杆菌的应用改善了玉米青贮的微生物谱，降低了乙醇产量，即使在青贮后70天捕获的分离株中没有被鉴定出来。亮点:乳酸菌在发酵第7天发育明显。植物乳杆菌在70天占优势，占乳酸菌总数的93%。随着乙醇产量的降低，布氏乳杆菌的微生物学特征得到改善。

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Revista de la Facultad de Ciencias Agrarias UNCuyo

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