The influence of Pseudomonas Fluorescens Bacteria and substances of stimulating nature on the productivity of the soybean plant and the damage of seeds by pathogens

Abstract

Soybeans are a strategic legume for global agriculture and play an important role in ensuring the food security of many countries. At the same time, biotic factors, in particular pathogens, prevent high and high-quality crop yields. Their control is carried out using various methods and techniques. However, with an excessive increase in the use of chemical pesticides, the ecological situation in agrocenoses becomes more complicated. Therefore, it is important to reduce the pesticide load in crop production technologies. The aim of the research is to evaluate the influence of Pseudomonas fluorescens bacteria and substances of stimulating nature on the productivity of soybean plants and seed damage by pathogens. The experiments were performed in the conditions of the Ukrainian Plant Quarantine Research Station of the Institute of Plant Protection of NAAS. The soil of the experimental plot is gray forest podzolic heavy loam. Xenia soybean variety was used for research. Field research was performed according to generally accepted methods. Soybean crops were sprayed during the growing season in the following periods: true trifoliate leaf, flowering and bean formation. For the treatment of soybean plants used a preparation based on bacteria Pseudomonas fluorescens with a titer of 3 x 109 CFU/cm3 with a rate of 5.0 l/ha when used in combination with substances with stimulant action. Over the years of research, the amount of precipitation (in some months of the growing season of soybean plants) was higher than the average monthly long-term norm. Pre-harvest desiccation of crops was not performed. Phytopathological examination of seeds obtained from soybean plants under different treatments was carried out according to DSTU 4138-2002. Studies have shown a positive effect of the drug based on P. fluorescens and substances of a stimulating nature on the biometric parameters of soybean plants and their productivity. In particular, the weight of 1000 seeds in the variants with spraying of plants increased in the range from 16 to 66 g. There was also a decrease in infection of soybean seeds with pathogens of fusarium wilt, Alternaria and penicillosis - fungi Fusarium oxysporum Schlecht, Alternaria spp. and Penicillium expansum Link. The study of immunoprotective and stimulating effects of combinations of biocomplexes is promising for the development of environmentally friendly measures to increase soybean yield and reduce seed damage by pathogens.