Phenol Removal by Tyrosinase, Immobilized in Modified Poly-N-vinylpyrrolidone, with Inorganic Coagulants Application

I. Romanovska, Yu. A. Shesterenko, O. Sevast’yanov, V. Osetrov, I. Pashkin
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Abstract

Enzymatic removal of phenol from aqueous solution was undertaken by the combined use of partially purified mushroom tyrosinase, immobilized in modified poly-N-vinylpyrrolidone, and inorganic coagulants (potassium, ammonium and ferric ammonium alum). Under optimum conditions (pH=6.5, T=25 °C, time of transformation – 1h) immobilized tyrosinase catalyzed the complete oxidation of phenol in batch reactor during 8 cycles, and the total number of cycles with a high degree of phenol transformation reaches 15. Inorganic coagulants eliminate colored products of phenol oxidation in a broad range of initial pollutant concentrations (0.5-10 mmol/dm3). The removal rates in all cases exceeded 97%
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改性聚n -乙烯基吡咯烷酮固定化酪氨酸酶对苯酚的去除及无机混凝剂的应用
通过将部分纯化的蘑菇酪氨酸酶固定在改性聚n -乙烯基吡咯烷酮和无机混凝剂(钾、铵和铁铵明矾)中,对水溶液中的苯酚进行酶解。在最佳条件(pH=6.5, T=25℃,转化时间- 1h)下,固定化酪氨酸酶催化间歇式反应器中苯酚的完全氧化需要8个循环,苯酚转化率较高的总循环次数达到15次。无机混凝剂可以在很宽的初始污染物浓度范围内(0.5-10 mmol/dm3)消除酚氧化的有色产物。所有病例的去除率均超过97%
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