I. Romanovska, Yu. A. Shesterenko, O. Sevast’yanov, V. Osetrov, I. Pashkin
{"title":"Phenol Removal by Tyrosinase, Immobilized in Modified Poly-N-vinylpyrrolidone, with Inorganic Coagulants Application","authors":"I. Romanovska, Yu. A. Shesterenko, O. Sevast’yanov, V. Osetrov, I. Pashkin","doi":"10.15866/IREBIC.V2I2.1511","DOIUrl":null,"url":null,"abstract":"Enzymatic removal of phenol from aqueous solution was undertaken by the combined use of partially purified mushroom tyrosinase, immobilized in modified poly-N-vinylpyrrolidone, and inorganic coagulants (potassium, ammonium and ferric ammonium alum). Under optimum conditions (pH=6.5, T=25 °C, time of transformation – 1h) immobilized tyrosinase catalyzed the complete oxidation of phenol in batch reactor during 8 cycles, and the total number of cycles with a high degree of phenol transformation reaches 15. Inorganic coagulants eliminate colored products of phenol oxidation in a broad range of initial pollutant concentrations (0.5-10 mmol/dm3). The removal rates in all cases exceeded 97%","PeriodicalId":14377,"journal":{"name":"International Review of Biophysical Chemistry","volume":"76 1","pages":"54-60"},"PeriodicalIF":0.0000,"publicationDate":"2011-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Review of Biophysical Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15866/IREBIC.V2I2.1511","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Enzymatic removal of phenol from aqueous solution was undertaken by the combined use of partially purified mushroom tyrosinase, immobilized in modified poly-N-vinylpyrrolidone, and inorganic coagulants (potassium, ammonium and ferric ammonium alum). Under optimum conditions (pH=6.5, T=25 °C, time of transformation – 1h) immobilized tyrosinase catalyzed the complete oxidation of phenol in batch reactor during 8 cycles, and the total number of cycles with a high degree of phenol transformation reaches 15. Inorganic coagulants eliminate colored products of phenol oxidation in a broad range of initial pollutant concentrations (0.5-10 mmol/dm3). The removal rates in all cases exceeded 97%