Seroprevalence of Brucellosis among Pigs of Commercial Farms in Chitwan District of Nepal

Kiran Pokhrel, Sulekha Sharma, Supriya Sharma, S. Adhikari, I. Dhakal, B. Devkota
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Abstract

Objectives: This study aimed to determine the seroprevalence of brucellosis among pigs of commercial farms in Chitwan district of Nepal. Methods: This cross sectional prospective study was conducted among 100 pigs of commercial farms located in western region of Chitwan district of Nepal. Blood sample was collected from each pig by the trained veterinarians and serum was extracted. Each serum sample was processed for Rose Bengal plate test (RBPT) and ELISA for the detection of Brucella spp. Data was analyzed using SPSS software version 21.0 and a p-value of less than 0.05 was considered as significant. Results: Prevalence of brucellosis in pigs was found to be 15% (15/100) by RBPT and 10% (10/100) by ELISA. There was statistically insignificant difference (p=0.98) in gender wise prevalence of brucellosis among pigs. Younger pigs below one year of age were more susceptible to brucellosis than the older pigs. Landrace breed showed more positive test results compared to other breeds. Conclusion: Pigs can be the potential source of transmission of brucellosis to humans. Considering the high economic loss on livestock sector and possible transmission to humans, awareness program and appropriate control strategies is warranted. Breed and age factors should be considered while adopting the control measures of brucellosis among pigs.
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尼泊尔奇旺地区商业农场猪的布鲁氏菌病血清流行率
目的:本研究旨在确定在尼泊尔奇旺地区的商业农场的猪布鲁氏菌病的血清患病率。方法:本横断面前瞻性研究在尼泊尔奇旺地区西部地区的100头商业农场进行。由训练有素的兽医采集每头猪的血液样本并提取血清。各血清标本经玫瑰孟加拉平板试验(RBPT)和酶联免疫吸附试验(ELISA)检测布鲁氏菌,数据采用SPSS 21.0软件进行分析,p值小于0.05为显著性差异。结果:猪布鲁氏菌病的RBPT法和ELISA法分别为15%(15/100)和10%(10/100)。猪布鲁氏菌病男女患病率差异无统计学意义(p=0.98)。一岁以下的幼猪比年长的猪更容易感染布鲁氏菌病。与其他品种相比,长白猪品种的阳性检测结果更多。结论:猪可能是布鲁氏菌病传播给人类的潜在来源。考虑到对畜牧业造成的巨大经济损失以及可能传播给人类,有必要开展宣传规划和采取适当的控制策略。在采取猪布鲁氏菌病控制措施时应考虑品种和年龄因素。
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