Investigation of the Interaction between Fat body and Ovary Development during Pupal Transformation in Silkworm, Bombyx mori

Esen Poyraz, E. Goncu, O. Parlak
{"title":"Investigation of the Interaction between Fat body and Ovary Development during Pupal Transformation in Silkworm, Bombyx mori","authors":"Esen Poyraz, E. Goncu, O. Parlak","doi":"10.18478/IUFSJB.04765","DOIUrl":null,"url":null,"abstract":"The insect fat body is the major biosynthetic and storage organ involved in lipid, carbohydrate, amino acid and nitrogen metabolism and protein synthesis. It also actively participates in vitellogenesis and ovary development, synthesizing the soluble precursor for yolk, i.e., vitellogenin in stage specific manner. The continual substance exchange among fat body, hemolymph and ovary is controlled by hormones, ecdysteroids and juvenile hormone, during metamorphosis of Bombyx mori (L.). The aim of the present study was to clarify the interactions between major proteins of fat body and hemolymph and their effect on ovarian development under hormonal factors during pupal-adult transformation. Detected fat body, hemolymph and ovary proteins are grouped as follows by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE): storage proteins (72 kDa and 76 kDa), 30 kDa proteins, ApoLp-I (230-250 kDa), egg specific protein (72 kDa and 64 kDa) and vitellogenins (178 kDa). Our results suggest that changes in the well-defined and characterized protein fractions quality and quantity of the fat body had a direct effect under hormonal factors on the ovary and egg development during metamorphosis.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"IUFS Journal of Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18478/IUFSJB.04765","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The insect fat body is the major biosynthetic and storage organ involved in lipid, carbohydrate, amino acid and nitrogen metabolism and protein synthesis. It also actively participates in vitellogenesis and ovary development, synthesizing the soluble precursor for yolk, i.e., vitellogenin in stage specific manner. The continual substance exchange among fat body, hemolymph and ovary is controlled by hormones, ecdysteroids and juvenile hormone, during metamorphosis of Bombyx mori (L.). The aim of the present study was to clarify the interactions between major proteins of fat body and hemolymph and their effect on ovarian development under hormonal factors during pupal-adult transformation. Detected fat body, hemolymph and ovary proteins are grouped as follows by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE): storage proteins (72 kDa and 76 kDa), 30 kDa proteins, ApoLp-I (230-250 kDa), egg specific protein (72 kDa and 64 kDa) and vitellogenins (178 kDa). Our results suggest that changes in the well-defined and characterized protein fractions quality and quantity of the fat body had a direct effect under hormonal factors on the ovary and egg development during metamorphosis.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
家蚕蛹转化过程中脂肪体与卵巢发育相互作用的研究
昆虫脂肪体是主要的生物合成和储存器官,参与脂质、碳水化合物、氨基酸和氮的代谢以及蛋白质的合成。它还积极参与卵黄发生和卵巢发育,以特定阶段的方式合成卵黄可溶性前体,即卵黄原蛋白。家蚕变态过程中脂肪体、血淋巴和卵巢之间持续的物质交换受激素、表皮甾体激素和幼体激素的控制。本研究旨在阐明在激素作用下,蛹-成虫转化过程中脂肪体主要蛋白与血淋巴的相互作用及其对卵巢发育的影响。采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)将检测到的脂肪体、血淋巴和卵巢蛋白分类为:储存蛋白(72 kDa和76 kDa)、30 kDa蛋白、ApoLp-I (230 ~ 250 kDa)、鸡蛋特异性蛋白(72 kDa和64 kDa)和卵黄原蛋白(178 kDa)。我们的研究结果表明,在激素作用下,脂肪体中定义明确和特征化的蛋白质组分、质量和数量的变化对变态过程中卵巢和卵子的发育有直接影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Lipoxygenase Inhibitory Activities of Some Plant Extracts and Chemical Compounds Three wheat cultivars’ responses to NaCl treatments: changes in lipid peroxidation, cell viability, hydrogen peroxide content and antioxidant defence parameters The role of Escherichia coli bacterioferritin in stressed induced conditions Phenolic composition and antibacterial activity of crude methanolic Calendula officinalis flower extract against plant pathogenic bacteria Scale Surface Microstructure and Scale Size in Three Mugilid Fishes (Teleostei, Mugilidae) of Iran from Three Different Habitats
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1