The Assessment of in Vitro Cardiotoxic Potentials for Synthetic Cannabinoid, AM-2201

Esra Nişikli, M. Akar, G. Ozhan
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Abstract

DOI : 10.26650/IstanbulJPharm.2019.19004 Synthetic cannabinoid abuse becomes more common in recent years, although knowledge about the risk of the relatively new synthetic cannabinoid molecules is not adequate. Data is mainly limited with new analytical methods and case reports related to their clinical effects. The studies are generally related to the cardiac effects of first defined molecules rather than every molecule in the group. The cardiac clinical effects of synthetic cannabinoid abuse and underlying mechanisms are not certain. Therefore, we aimed to investigate AM-2201, one of synthetic cannabinoids, because knowledge for AM-2201 is less than the others in this group. The cardiotoxicity and underlying mechanisms of AM-2201 were assessed on cardiac cell culture. The half-maximal inhibition concentration (IC50) values were 101.49 and 63.33 M by WST-1 and LDH assays. AM-2201 was not induced either the total antioxidant capacity (TAC) or reactive oxygen species (ROS) levels. As to the measurements Annexin V-FITC and acridine orange dye, AM-2201 did not induce apoptosis and the primary cell death was necrosis. According to our results, further studies such as mechanism on cell death and cancer pathways should be investigated. You may cite this article as : Nisikli E, Celiksoz Akar M, Ozhan G (2019). The Assessment of in Vitro Cardiotoxic Potentials for Synthetic Cannabinoid, AM-2201. Istanbul J Pharm 10.26650/IstanbulJPharm.2019.19004.
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合成大麻素AM-2201体外心脏毒性电位的评估
近年来,合成大麻素滥用变得越来越普遍,尽管对相对较新的合成大麻素分子的风险了解并不充分。数据主要局限于新的分析方法和与临床效果相关的病例报告。这些研究通常与第一个确定的分子的心脏作用有关,而不是与组中的每个分子有关。滥用合成大麻素对心脏的临床影响及其潜在机制尚不确定。因此,我们的目标是研究合成大麻素之一AM-2201,因为对AM-2201的了解比该组的其他大麻素少。在心脏细胞培养中评估AM-2201的心脏毒性及其潜在机制。WST-1和LDH的半最大抑制浓度(IC50)分别为101.49和63.33M。AM-2201不诱导总抗氧化能力(TAC)和活性氧(ROS)水平。Annexin V-FITC和吖啶橙染色检测显示,AM-2201不诱导细胞凋亡,细胞以坏死为主死亡。根据我们的研究结果,需要进一步研究细胞死亡机制和癌症途径。你可以将这篇文章引用为:Nisikli E, Celiksoz Akar M, Ozhan G(2019)。合成大麻素AM-2201体外心脏毒性电位的评估IstanbulJPharm 10.26650/ istanbuljpharma .2019.19004。
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