NITROGEN DETERMINATION IN WHEAT LEAVES

D. Roberts
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引用次数: 2

Abstract

A survey of the more promising modifications of the Kjeldahl method for nitrogen determination indicates that the method using mercuric oxide as the catalyst as recommended by the Association of Official Agricultural Chemists is the most satisfactory. However small discrepancies were found when this method was applied to wheat leaves fractionated into soluble and protein (actually coagulable and indiffusible) nitrogen fractions. This method includes all the nitrate nitrogen in wheat leaves together with the organic nitrogen even when no pretreatment with salicylic acid is used. A simple and satisfactory method of separating coagulable and indiffusible nitrogen from soluble nitrogen in wheat leaves and seedlings is described.
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小麦叶片氮含量测定
一项对凯氏定氮法更有前途的改进的调查表明,由官方农业化学家协会推荐的使用氧化汞作为催化剂的方法是最令人满意的。然而,当这种方法应用于小麦叶片分解成可溶性和蛋白质(实际上是凝固和不扩散)氮组分时,发现差异很小。该方法包括小麦叶片中所有的硝态氮和有机氮,即使不使用水杨酸预处理。介绍了一种从小麦叶片和幼苗中分离混凝态氮和不扩散态氮和可溶性氮的简便方法。
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