Enhancing Neurological Competence of Nanoencapsulated Cordyceps/Turmeric Extracts in Human Neuroblastoma SH-SY5Y Cells.

IF 2.3 4区医学 Q3 BIOPHYSICS Cellular and molecular bioengineering Pub Date : 2022-12-13 eCollection Date: 2023-02-01 DOI:10.1007/s12195-022-00752-w

Palakorn Kaokaen, Natchadaporn Sorraksa, Ruchee Phonchai, Nipha Chaicharoenaudomrung, Phongsakorn Kunhorm, Parinya Noisa

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引用次数: 2

Abstract

Introduction: Neurological diseases, including Alzheimer's, Parkinson's diseases, and brain cancers, are reportedly caused by genetic aberration and cellular malfunction. Herbs with bioactive compounds that have anti-oxidant effects such as cordyceps and turmeric, are of interest to clinical applications due to their minimal adverse effects. The aim of study is to develop the nanoencapsulated cordyceps and turmeric extracts and investigate their capability to enhance the biological activity and improve neuronal function.

Methods: Human neuroblastoma SH-SY5Y cells were utilized as a neuronal model to investigate the properties of nanoencapsulated cordyceps or turmeric extracts, called CMP and TEP, respectively. SH-SY5Y cells were treated with either CMP or TEP and examined the biological consequences, including neuronal maturation and neuronal function.

Results: The results showed that both CMP and TEP improved cellular uptake efficiency within 6 h by 2.3 and 2.8 times, respectively. Besides, they were able to inhibit cellular proliferation of SH-SY5Y cells up to 153- and 218-fold changes, and increase the expression of mature neuronal markers (TUJ1, PAX6, and NESTIN). Upon the treatment of CMP and TEP, the expression of dopaminergic-specific genes (LMX1B, FOXA2, EN1, and NURR1), and the secretion level of dopamine were significantly improved up to 3.3-fold and 3.0-fold, respectively, while the expression of Alzheimer genes (PSEN1, PSEN2, and APP), and the secretion of amyloid precursor protein were significantly reduced by 32-fold and 108-fold, respectively. Importantly, the autophagy activity was upregulated by CMP and TEP at 6.3- and 5.5-fold changes, respectively.

Conclusions: This finding suggested that the nanoencapsulated cordyceps and turmeric extracts accelerated neuronal maturation and alleviated neuronal pathology in human neural cells. This paves the way for nanotechnology-driven drug delivery systems that could potentially be used as an alternative medicine in the future for neurological diseases.

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纳米虫草/姜黄提取物增强人神经母细胞瘤 SH-SY5Y 细胞的神经能力

导言：据报道，包括阿尔茨海默氏症、帕金森氏症和脑癌在内的神经系统疾病是由基因畸变和细胞功能失调引起的。冬虫夏草和姜黄等草药具有抗氧化作用的生物活性化合物，因其不良反应极小而受到临床应用的关注。研究目的是开发纳米虫草和姜黄提取物，并研究其增强生物活性和改善神经元功能的能力。方法：以人神经母细胞瘤 SH-SY5Y 细胞为神经元模型，研究分别称为 CMP 和 TEP 的纳米虫草或姜黄提取物的特性。用 CMP 或 TEP 处理 SH-SY5Y 细胞，并研究其生物学后果，包括神经元成熟和神经元功能：结果表明，CMP 和 TEP 在 6 小时内分别将细胞吸收效率提高了 2.3 倍和 2.8 倍。此外，它们还能抑制 SH-SY5Y 细胞的增殖，使增殖倍数分别达到 153 倍和 218 倍，并增加成熟神经元标志物（TUJ1、PAX6 和 NESTIN）的表达。经 CMP 和 TEP 处理后，多巴胺能特异基因（LMX1B、FOXA2、EN1 和 NURR1）的表达和多巴胺的分泌水平分别显著提高了 3.3 倍和 3.0 倍，而阿尔茨海默基因（PSEN1、PSEN2 和 APP）的表达和淀粉样前体蛋白的分泌则分别显著降低了 32 倍和 108 倍。重要的是，CMP和TEP分别以6.3倍和5.5倍的变化上调了自噬活性：这一发现表明，纳米虫草和姜黄提取物可加速神经元成熟，缓解人类神经细胞的神经元病理变化。这为纳米技术驱动的给药系统铺平了道路，该系统未来有可能被用作治疗神经系统疾病的替代药物。

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来源期刊

Cellular and molecular bioengineering 生物-生物物理

CiteScore

5.60

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： The field of cellular and molecular bioengineering seeks to understand, so that we may ultimately control, the mechanical, chemical, and electrical processes of the cell. A key challenge in improving human health is to understand how cellular behavior arises from molecular-level interactions. CMBE, an official journal of the Biomedical Engineering Society, publishes original research and review papers in the following seven general areas: Molecular: DNA-protein/RNA-protein interactions, protein folding and function, protein-protein and receptor-ligand interactions, lipids, polysaccharides, molecular motors, and the biophysics of macromolecules that function as therapeutics or engineered matrices, for example. Cellular: Studies of how cells sense physicochemical events surrounding and within cells, and how cells transduce these events into biological responses. Specific cell processes of interest include cell growth, differentiation, migration, signal transduction, protein secretion and transport, gene expression and regulation, and cell-matrix interactions. Mechanobiology: The mechanical properties of cells and biomolecules, cellular/molecular force generation and adhesion, the response of cells to their mechanical microenvironment, and mechanotransduction in response to various physical forces such as fluid shear stress. Nanomedicine: The engineering of nanoparticles for advanced drug delivery and molecular imaging applications, with particular focus on the interaction of such particles with living cells. Also, the application of nanostructured materials to control the behavior of cells and biomolecules.