Effect of different doses of recombinant human bone morphogenetic protein 2 on osteogenic activities of human osteosarcoma cell line SaOS-2

Q3 Medicine 中华地方病学杂志 Pub Date : 2011-05-20 DOI:10.3760/CMA.J.ISSN.1000-4955.2011.03.010

Huo Liang-liang, Liu Kang-kang, Zhao Li-jun, Shi Yu-xia, Zhang Wei, Pei Junrui, Geng Li-bin, Gao Yanhui

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Abstract

Objective To investigate the effect of recombinant human bone morphogenetic protein 2 (rhBMP-2) on the osteogenic activities of human osteosarcoma cell line SaOS-2. Methods SaOS-2 cells were exposed to rhBMP-2 for 12,24,48 h at 0(control) ,2,20,200 μg/L, respectively. The mRNA expression of alkaline phosphatase(ALP) and bone gla(BCP) were detected by real time polymerase chain reaction. Results The mRNA expression of ALP and BGP of SaOS-2 cells increased gradually with rhBMP-2. The mRNA expression of ALP of the 20 μg/L group exposed for 48 h(1.60 ± 0.64), and the 200 μg/L group exposed for 12,48 h(1.70 ± 0.41, 1.80±0.19) were significantly higher than those of control (12 h: 0.80±0.25, 48 h: 0.74±0.21, allP<0.05). The mRNA expression of BGP of the 2 μg/L group exposed for 24 h(1.67 ± 0.33), the 20 μg/L group exposed for 12,24 h(2.42 ± 0.13,1.82 ± 0.14) and the 200 μg/L group exposed for 12,24 h(1.46 ± 0.11,1.24 ± 0.07) were significantly higher than those of control( 12 h: 1.01 ± 0.14, 24 h: 0.84 ± 0.12, all P< 0.05). Conclusions rhBMP-2 can promote the mRNA expression of ALP and BGP of SaOS-2 cells. They have a dose-response relationship, but represent a different dose-response effect. Key words: Bone morphogenetic proteins; Osteogenesis; Alkaline phosphatase; Osteocalcin

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不同剂量重组人骨形态发生蛋白2对人骨肉瘤细胞系SaOS-2成骨活性的影响

目的探讨重组人骨形态发生蛋白2 (rhBMP-2)对人骨肉瘤细胞系SaOS-2成骨活性的影响。方法分别以0(对照)、2、20、200 μg/L浓度的rhBMP-2作用于SaOS-2细胞12、24、48 h。实时聚合酶链反应检测碱性磷酸酶(ALP)和骨玻璃(BCP) mRNA表达。结果SaOS-2细胞ALP、BGP mRNA表达随rhBMP-2的增加而逐渐升高。20 μg/L暴露48 h组ALP mRNA表达量(1.60±0.64)和200 μg/L暴露12、48 h组ALP mRNA表达量(1.70±0.41、1.80±0.19)均显著高于对照组(12 h: 0.80±0.25、48 h: 0.74±0.21，均p <0.05)。2 μg/L暴露24 h组(1.67±0.33)、20 μg/L暴露12、24 h组(2.42±0.13,1.82±0.14)和200 μg/L暴露12、24 h组(1.46±0.11,1.24±0.07)的BGP mRNA表达量均显著高于对照组(12 h: 1.01±0.14,24 h: 0.84±0.12，均P< 0.05)。结论rhBMP-2可促进SaOS-2细胞ALP和BGP mRNA的表达。它们具有剂量-反应关系，但代表不同的剂量-反应效应。关键词:骨形态发生蛋白;骨生成;碱性磷酸酶;骨钙素

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来源期刊

中华地方病学杂志我国对人类健康危害特别严重的地方性疾病：克山病、大骨节病、碘缺乏病、地方性氟中毒、地方性砷中毒、鼠疫、布鲁氏菌病、寄生虫、新冠肺炎等疾病，同时还报道多发性自然疫源性疾病。

CiteScore

1.60

自引率

0.00%

发文量

8714

期刊介绍： The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.