Determination of Quercetin in Soursop (Annona muricata Linn.) Leaves 10 ml Liquid Preparation Using UV-Spectroscopic Method

Abstract

Quercetin is a flavonoid compound that contains in Annona muricata L. leaves. UV Spectrophotometric method was used to determine quercetin from Annona muricata in liquid. The degradation was determined by selectivity, linearity, accuracy, precision, LOD, and LOQ. Selectivity showed that spectra have maximum absorbance at λ 374 nm. The good linearity was showed by y = 0,07368x - 0,01937 (r = 0,9984; p<0,05). The precision of standard quercetin in a concentration of 6.4 ppm showed a relative standard deviation (RSD) of 0.19%. LOD and LOQ of standard quercetin were 0.0240 µg/ml and 0.0801 µg/ml, and the accuracy was 91.867±7.59%. Determination of quercetin in Annona muricata leaf extract showed the concentration of 12.62±3.29% and (263.96 ± 6.69) mg in 10 ml liquid.