Morphological Characteristics and Growth Abilities of Free Living Amoeba Isolated From Domestic Tap Water Samples in İstanbul

Z. Zeybek, Miray Üstüntürk, Ali Rıza Binay
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引用次数: 6

Abstract

Free living amoeba (FLA) have been isolated from water and soil samples throughout the world. Among the many genera of FLA , members of only four genera are responsible for human disease: Acanthamoeba spp., Balamuthia mandrillaris , Naegleria fowleri and Sappinia diploidea . Naegleria spp. and Acanthamoeba spp. can be cultivated on nonnutrient agar (NNA) in the presence of living or dead bacteria. Balamuthia , however, will not grow with bacteria as a food source but they can feed upon smaller soil amoeba. In the current study we try to find out the morphological appearance (Trichrome staining) and growth characteristics of free living amoeba in different liquid culture media previously isolated from domestic tap water samples (10 isolates) and a strain of Acanthamoeba castellanii (A. castellanii) isolated from a keratitis case. Although A.castellanii strain grew on proteose peptone-yeast extract-glucose and proteose peptone-yeast (with or without antibiotics), our isolates from domestic tap water samples did not grow on any tested liquid culture media. It is suggested that investigating both the morphological characteristics and the growth characteristics on liquid culture media are necessary for the identification of free living amoeba.
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İstanbul生活自来水中分离的自由生活变形虫的形态特征和生长能力
自由生活的变形虫(FLA)已经从世界各地的水和土壤样品中分离出来。在FLA的许多属中,只有4个属的成员对人类疾病负责:棘阿米巴,曼氏巴拉姆西亚,福氏奈格里亚和Sappinia diploidea。Naegleria spp.和棘阿米巴spp.可以在非营养性琼脂(NNA)上培养,存在活的或死的细菌。然而,Balamuthia不会以细菌为食物来源,但它们可以以较小的土壤变形虫为食。在本研究中,我们试图了解从生活自来水样品(10株)和从角膜炎病例中分离的一株卡斯特兰棘阿米巴(a . castellanii)分离的不同液体培养基中自由活阿米巴的形态外观(三色染色)和生长特征。虽然castellanii菌株在蛋白酶-酵母提取物-葡萄糖和蛋白酶-酵母(含或不含抗生素)上生长,但我们从生活自来水样品中分离的菌株在任何测试的液体培养基上都没有生长。因此,研究其形态特征和在液体培养基上的生长特征是鉴定自由生活变形虫的必要条件。
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