Pressure Effects on G-Quadruplex Structures Stabilized by Two Different Counter-Ions

E. J. Baldassarri, M. G. Ortore, C. Ferrero, S. Finet, F. Spinozzi, P. Mariani
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Abstract

It is well known that the formation of G-quadruplex by guanosine derivatives strongly depends on counter-ions. To investigate quadruplex stability in the presence of different counter-ions and in the absence of a covalent axial backbone, high-pressure X-ray diffraction experiments have been performed on lyotropic phases of guanosine 5’-monophosphate (GMP) in the form of sodium and ammonium salts. As a result, concentration-pressure phase diagrams were obtained in a pressure range from 1 bar to about 2 kbar, and the structural properties of the different phases derived. Interestingly, cholesteric (Ch) and hexagonal (H) columnar phases were found in both GMP salts, but during compression a reverse behavior was detected: in GMP sodium salt, pressure induces a H-Ch phase transition, while in GMP ammonium salt the induced transition is Ch-H. Different counter-ion stabilization, which control quadruplex length and the consequence of lateral interactions, probably explain such observation
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两种不同反离子稳定g -四元结构的压力效应
众所周知,鸟苷衍生物的g -四重体的形成强烈依赖于反离子。为了研究在不同反离子存在和缺少共价轴主链的情况下,鸟苷5′-单磷酸鸟苷(GMP)在钠盐和铵盐形式下的四重相稳定性,进行了高压x射线衍射实验。得到了在1 ~ 2 kbar压力范围内的浓度-压力相图,并得到了不同相的结构特性。有趣的是,在两种GMP盐中都发现了胆甾(Ch)和六方(H)柱状相,但在压缩过程中发现了相反的行为:在GMP钠盐中,压力诱导H-Ch相转变,而在GMP铵盐中诱导的转变是Ch-H相。不同的反离子稳定性控制着四重链长度和横向相互作用的结果,可能解释了这种观察结果
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