Fields of application and safe management of nuclear magnetic resonance spectroscopy in biomedicine for non-medical purposes

Abstract

Nuclear magnetic resonance (NMR) spectroscopy has assumed a leading position among the instrumental methods used in the analysis and characterization of organic and non-organic molecules, in addition to the application of 13C and 1H high-resolution Fourier transform techniques for structural analysis of lipid components, peptides and proteins and carbohydrates. Thanks to the increasingly higher magnetic fields of the spectrometers, there has been a significant increase in resolution which has allowed us to move on to larger molecules and increasingly sophisticated techniques. Today multidimensional spectra such as the correlation spectra between 13C, 1H and 15N are routine analysis whose most common acronyms are FOCSY, NOESY and TROSY. Most of these experiments are based on the NOE (Nuclear Overhauser Effect) phenomenon which provides information on the dipolar interaction between magnetic nuclei. We will also deal with the various types of installations in relation to the safety standards that these equipment require for correct use.