Gene Expression of OCT4 and NANOG Correlated with Advanced Stage and Worse Survival in Breast Cancer Patients

Fawziya A. R. Ibrahim, S. E. E. Feky, Kadhim K. Kadhim, N. A. E. Moneim, Mohammad Abdel-Rahman Ahmmad, S. Sheweita
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Abstract

The present study aimed to show the correlation between expression of cancer stem cell markers (OCT4 and NANOG) with both clinicopathological features and survival of breast cancer (BC) patients. Methods: The gene expressions of OCT4 and NANOG were quantified using real time polymerase chain reaction, clinicopathological data have been collected from patients' data records and patients were followed-up with a median duration of 110 months. Results: OCT4 (p<0.001), and NANOG (p<0.001) expressions were upregulated in BC tissues compared to adjacent normal tissues. OCT4 and NANOG were associated with poor histological grade (p=0.029, 0.025) and advanced clinical stage (p=0.001, 0.042 respectively). OCT4 alone showed a significant association with lymph nodes involvement (p=0.006), metastasis (p=0.024) and was significantly correlated to patients' age (p=0.009). NANOG also showed a significant positive correlation with ERα and PR receptors expression (p=0.004 and 0.005 respectively). Kaplan–Meier curves disclosed that NANOG (p=0.028, 0.050) positive expression was associated with worse DFS and OS, while OCT4 (p=0.200, 0.205) was correlated with poor DFS and OS but not significant statistically. Univariate analysis using Cox proportional hazards regression model analysis showed that OCT4 (p = 0.002), NANOG (p = 0.021), and ERα status (p = 0.004) had significant predictive values for poor DFS. However, the multivariate analysis did not show that any of them can be used as independent prognostic markers for DSF. Conclusions: From these findings, it may be concluded that the upregulated expressions of OCT4 and NANOG were associated with worse clinical outcome and could be used as predictive markers for poor DFS in BC patients.
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OCT4和NANOG基因表达与乳腺癌患者晚期和较差生存率相关
本研究旨在揭示肿瘤干细胞标志物(OCT4和NANOG)的表达与乳腺癌(BC)患者的临床病理特征和生存之间的相关性。方法:采用实时聚合酶链反应定量检测OCT4和NANOG基因表达,收集患者资料记录临床病理资料,对患者进行中位随访110个月。结果:与邻近正常组织相比,BC组织中OCT4 (p<0.001)和NANOG (p<0.001)表达上调。OCT4和NANOG与组织学分级差(p=0.029, 0.025)和临床分期晚(p=0.001, 0.042)相关。单独OCT4与淋巴结累及(p=0.006)、转移(p=0.024)显著相关,与患者年龄显著相关(p=0.009)。NANOG与ERα和PR受体表达也呈显著正相关(p分别为0.004和0.005)。Kaplan-Meier曲线显示NANOG (p=0.028, 0.050)阳性表达与较差的DFS和OS相关,OCT4 (p=0.200, 0.205)阳性表达与较差的DFS和OS相关,但统计学意义不显著。单因素分析采用Cox比例风险回归模型分析显示,OCT4 (p = 0.002)、NANOG (p = 0.021)和ERα状态(p = 0.004)对不良DFS有显著的预测价值。然而,多变量分析并没有显示其中任何一个可以作为DSF的独立预后指标。结论:从这些发现可以得出结论,OCT4和NANOG的表达上调与较差的临床预后相关,可以作为BC患者不良DFS的预测指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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