Angiotensin II Induces Differentiation of Human Neuroblastoma Cells by Increasing MAP2 and ROS Levels.

IF 2.1 4区 医学 Q3 PERIPHERAL VASCULAR DISEASE Journal of the Renin-Angiotensin-Aldosterone System Pub Date : 2021-01-01 DOI:10.1155/2021/6191417
Bryan Jael Collazo, Dariana Morales-Vázquez, Jaylene Álvarez-Del Valle, Javier E Sierra-Pagan, Juan Carlos Medina, Jarold Méndez-Álvarez, Yamil Gerena
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引用次数: 1

Abstract

Introduction: The roles of angiotensin II (Ang II) in the brain are still under investigation. In this study, we investigated if Ang II influences differentiation of human neuroblastoma cells with simultaneous activation of NADPH oxidase and reactive oxygen species (ROS). Moreover, we investigated the Ang II receptor type involved during differentiation.

Methods: Human neuroblastoma cells (SH-SY5Y; 5 × 105 cells) were exposed to Ang II (600 nM) for 24 h. Differentiation was monitored by measuring MAP2 and NF-H levels. Cell size and ROS were analyzed by flow cytometry, and NADPH oxidase activation was assayed using apocynin (500 μM). Ang II receptors (ATR) activation was assayed using ATR blockers or Ang II metabolism inhibitors (10-7 M).

Results: (1) Cell size decreased significantly in Ang II-treated cells; (2) MAP2 and ROS increased significantly in Ang II-treated cells with no changes in viability; (3) MAP2 and ROS decreased significantly in cells incubated with Ang II plus apocynin. (4) A significant decrease in MAP2 was observed in cells exposed to Ang II plus PD123.319 (AT2R blocker).

Conclusion: Our findings suggest that Ang II influences differentiation of SH-SY5Y by increasing MAP2 through the AT2R. The increase in MAP2 and ROS were also mediated through NADPH oxidase with no cell death.

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血管紧张素II通过增加MAP2和ROS水平诱导人神经母细胞瘤细胞分化
血管紧张素II (Ang II)在大脑中的作用仍在研究中。在这项研究中,我们研究了Ang II是否通过同时激活NADPH氧化酶和活性氧(ROS)来影响人神经母细胞瘤细胞的分化。此外,我们还研究了在分化过程中参与的Ang II受体类型。方法:人神经母细胞瘤细胞(SH-SY5Y;5 × 105个细胞)暴露于Ang II (600 nM) 24h。通过测量MAP2和NF-H水平来监测分化。流式细胞术检测细胞大小和ROS, 500 μM夹竹桃碱检测NADPH氧化酶活性。使用ATR阻滞剂或Ang II代谢抑制剂(10-7 M)检测Ang II受体(ATR)的激活。结果:(1)Angⅱ处理后细胞大小明显减小;(2)在angii处理的细胞中,MAP2和ROS显著升高,但细胞活力没有变化;(3) angii + apocynin孵育的细胞中,MAP2和ROS显著降低。(4)暴露于Ang II + PD123.319 (AT2R阻滞剂)的细胞中,MAP2显著降低。结论:我们的研究结果表明Ang II通过AT2R增加MAP2影响SH-SY5Y的分化。MAP2和ROS的增加也通过NADPH氧化酶介导,无细胞死亡。
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来源期刊
CiteScore
6.20
自引率
0.00%
发文量
16
审稿时长
6-12 weeks
期刊介绍: JRAAS is a peer-reviewed, open access journal, serving as a resource for biomedical professionals, primarily with an active interest in the renin-angiotensin-aldosterone system in humans and other mammals. It publishes original research and reviews on the normal and abnormal function of this system and its pharmacology and therapeutics, mostly in a cardiovascular context but including research in all areas where this system is present, including the brain, lungs and gastro-intestinal tract.
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