Engineered probiotics as live biotherapeutics for diagnosis and treatment of human diseases.

IF 6 2区生物学 Q1 MICROBIOLOGY Critical Reviews in Microbiology Pub Date : 2024-05-01 Epub Date: 2023-03-22 DOI:10.1080/1040841X.2023.2190392

Jiao Meng, Shufan Liu, Xin Wu

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Abstract

The use of probiotics to regulate the intestinal microbiota to prevent and treat a large number of disorders and diseases has been an international research hotspot. Although conventional probiotics have a certain regulatory role in nutrient metabolism, inhibiting pathogens, inducing immune regulation, and maintaining intestinal epithelial barrier function, they are unable to treat certain diseases. In recent years, aided by the continuous development of synthetic biology, engineering probiotics with desired characteristics and functionalities to benefit human health has made significant progress. In this article, we summarise the mechanism of action of conventional probiotics and their limitations and highlight the latest developments in the design and construction of probiotics as living diagnostics and therapeutics for the detection and treatment of a series of diseases, including pathogen infections, cancer, intestinal inflammation, metabolic disorders, vaccine delivery, cognitive health, and fatty liver. Besides we discuss the concerns regarding engineered probiotics and corresponding countermeasures and outline the desired features in the future development of engineered live biotherapeutics.

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将工程益生菌作为诊断和治疗人类疾病的活生物疗法。

利用益生菌调节肠道微生物群来预防和治疗大量疾病一直是国际研究热点。传统的益生菌虽然在营养代谢、抑制病原体、诱导免疫调节、维持肠道上皮屏障功能等方面具有一定的调节作用，但却无法治疗某些疾病。近年来，在合成生物学不断发展的推动下，具有理想特性和功能以造福人类健康的益生菌工程技术取得了重大进展。在这篇文章中，我们总结了传统益生菌的作用机理及其局限性，并重点介绍了设计和构建益生菌作为活体诊断和治疗手段用于检测和治疗病原体感染、癌症、肠道炎症、代谢紊乱、疫苗递送、认知健康和脂肪肝等一系列疾病的最新进展。此外，我们还讨论了对工程益生菌的担忧和相应对策，并概述了未来开发工程活生物疗法所需的特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical Reviews in Microbiology 生物-微生物学

CiteScore

14.70

自引率

0.00%

发文量

期刊介绍： Critical Reviews in Microbiology is an international, peer-reviewed journal that publishes comprehensive reviews covering all areas of microbiology relevant to humans and animals, including medical and veterinary microbiology, public health and environmental microbiology. These may include subjects related to microbial molecular biology, immunopathogenicity, physiology, biochemistry, structure, and epidemiology. Of particular interest are reviews covering clinical aspects of bacterial, virological, fungal and parasitic diseases. All reviews must be analytical, comprehensive, and balanced in nature. Editors welcome uninvited submissions, as well as suggested topics for reviews accompanied by an abstract.