Recomendaciones preanalíticas para la obtención y análisis de ADN circulante a partir de sangre periférica. Recomendación (2018)

Revista del Laboratorio Clínico Pub Date : 2019-07-01 DOI:10.1016/j.labcli.2018.11.002

Álvaro González , Clara Pérez Barrios , Hada Macher , Marta Sánchez-Carbayo , Antonio Barco Sánchez , Antonio Fernández Suárez , Antonio Martínez Peinado , Blanca Ortiz Muñoz , Jaume Trapé Pujol , José Diego Santotoribio Camacho , Josep María Augé Fradera , María Jesús Gaspar Blázquez , Rosa Carbonell Muñoz , Xavier Filella Pla , Comité Científico de la Sociedad Española de Medicina de Laboratorio (SEQCML)

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Abstract

Cell-free DNA analysis in peripheral blood has been shown to be useful in oncology, organ transplantation, and prenatal screening. For its introduction into the clinical laboratory, it is necessary to ensure appropriate, reproducible and standardised pre-analytical protocols are in place. The aim of this document is to provide pre-analytical recommendations for obtaining of cell free DNA from peripheral blood. These recommendations include the type of sample and extraction tube, the method of centrifugation, the method for cell free DNA extraction, and measurement and storage conditions.

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