Assessment of mold contamination in hurricane-damaged homes in Houston, Texas after sanitization by volunteers.

Stephen Vesper, Kevin G Libuit, Nicolas Esguerra, Andrew Cross
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Abstract

The purpose of this pilot study was to evaluate the effectiveness of mold sanitation in homes that suffered hurricane-related water damage. After a home is flooded, sanitation of the structure for mold is necessary before the interior of the home can be rebuilt. In this study, homes (n = 6) in Houston, Texas that had been flooded by Hurricane Harvey were sanitized by volunteers. At either 6, 8, 15, 25, 34, or 56 days after the sanitation was completed, a Button sampler was used to collect a 48-hour air sample, so that the mold cells in the air could be quantified. Each air sample was then analyzed by quantitative PCR (qPCR) assays for the 36 molds in the Environmental Relative Moldiness Index (ERMI) panel of indicator molds. Quantifying the 36-ERMI molds in air samples results in "ERMI-like" values. The ERMI-like values in the sanitized homes were inversely correlated (Pearson p - value 0.04) with the log of the number of days after the sanitation was completed, an indication that it takes time after sanitation for the mold levels to stabilize. This pilot study demonstrated that the ERMI-like metric was useful in assessing post-sanitation mold levels in previously flooded homes.

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在志愿者进行消毒后,对德克萨斯州休斯顿被飓风破坏的房屋进行霉菌污染评估。
这项试点研究的目的是评估在遭受飓风相关水害的家庭中霉菌卫生的有效性。房屋被淹后,在房屋内部重建之前,必须对结构进行防霉卫生。在这项研究中,志愿者对德克萨斯州休斯顿被哈维飓风淹没的房屋(n = 6)进行了消毒。在卫生处理完成后的6、8、15、25、34或56天,使用Button™取样器收集48小时空气样本,以便对空气中的霉菌细胞进行定量。然后对指示霉菌的环境相对发霉指数(ERMI)面板中的36个霉菌进行定量PCR (qPCR)分析。量化空气样品中的36-ERMI模具可得到“类似ermi”的值。消毒家庭的ERMI-like值与完成卫生后天数的对数呈负相关(Pearson p值为0.04),表明卫生后霉菌水平需要一段时间才能稳定。该试点研究表明,类似ermi的度量方法可用于评估以前被洪水淹没的房屋中卫生后的霉菌水平。
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