Engeletin ameliorates sevoflurane-induced cognitive impairment by activating PPAR-gamma in neonatal mice.

IF 1.3 4区医学 Q4 CLINICAL NEUROLOGY Neuropathology Pub Date : 2023-12-01 Epub Date: 2023-04-10 DOI:10.1111/neup.12905

Su Jiang, Ying Xiong, Xinyan Wang

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Abstract

Sevoflurane (SEV) is a commonly used anesthetic in pediatric surgery. Recent studies reported that repeated use of SEV contributes to cognitive impairment. Engeletin has been discovered to exert anti-inflammatory effects in various diseases. However, the detailed roles and mechanisms of engeletin in SEV-induced cognitive dysfunction of neonatal mice remain unclear. In this study, C57BL/6 neonatal mice were randomly divided into Ctrl, SEV, SEV + Engeletin (10 mg /kg), SEV + Engeletin (20 mg/kg), and SEV + Engeletin (40 mg/kg) groups. The Morris water maze (MWM) test suggested that engeletin treatment significantly improved SEV-induced cognitive impairment in neonatal mice. Employing ELISA and Nissl staining analysis, engeletin reduced neuroinflammation and loss of nerve cells caused by SEV, respectively. The treatment of engeletin dramatically suppressed the activation of microglia and apoptosis induced by SEV in the hippocampus of neonatal mice. Furthermore, the inhibition of PPAR-γ obviously reversed the abovementioned effects of engeletin in the hippocampus of newborn mice. In conclusion, this study verified that engeletin notably ameliorated SEV-induced cognitive deficiencies in neonatal mice at least partially by mediating the expression of PPAR-γ.

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eneletin通过激活新生小鼠ppar - γ改善七氟醚诱导的认知障碍。

七氟醚(SEV)是儿科手术中常用的麻醉剂。最近的研究报道，反复使用SEV会导致认知障碍。恩格列素已被发现对多种疾病有抗炎作用。然而，eneletin在sev诱导的新生小鼠认知功能障碍中的具体作用和机制尚不清楚。将C57BL/6新生小鼠随机分为Ctrl组、SEV组、SEV + Engeletin组(10 mg/kg)、SEV + Engeletin组(20 mg/kg)和SEV + Engeletin组(40 mg/kg)。Morris水迷宫(MWM)实验表明，engeletin治疗可显著改善sev诱导的新生小鼠认知功能障碍。ELISA和尼氏染色分析显示，恩格莱素分别减少了SEV引起的神经炎症和神经细胞损失。恩格列素处理可显著抑制SEV诱导的新生小鼠海马小胶质细胞的活化和凋亡。此外，抑制PPAR-γ明显逆转了新生小鼠海马中engeletin的上述作用。总之，本研究证实，eneletin至少部分通过介导PPAR-γ的表达，显著改善了sev诱导的新生小鼠认知缺陷。

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来源期刊

Neuropathology 医学-病理学

CiteScore

4.10

自引率

4.30%

发文量

105

审稿时长

6-12 weeks

期刊介绍： Neuropathology is an international journal sponsored by the Japanese Society of Neuropathology and publishes peer-reviewed original papers dealing with all aspects of human and experimental neuropathology and related fields of research. The Journal aims to promote the international exchange of results and encourages authors from all countries to submit papers in the following categories: Original Articles, Case Reports, Short Communications, Occasional Reviews, Editorials and Letters to the Editor. All articles are peer-reviewed by at least two researchers expert in the field of the submitted paper.