Katie M. Weiss, Sierra K. Kucko, Sahar Mokhtari, Timothy J. Keenan, Anthony William Wren
{"title":"Investigating the structure, solubility, and antibacterial properties of silver- and copper-doped hydroxyapatite","authors":"Katie M. Weiss, Sierra K. Kucko, Sahar Mokhtari, Timothy J. Keenan, Anthony William Wren","doi":"10.1002/jbm.b.35151","DOIUrl":null,"url":null,"abstract":"<p>Hydroxyapatite (<i>HA</i>) powders were synthesized by the wet precipitation method in which two experimental compositions were synthesized (10 mol% <i>Ag-HA</i> and <i>Cu-HA</i>) where the CaNO<sub>3</sub> content was partially substituted with AgNO<sub>3</sub> and Cu(NO<sub>3</sub>)<sub>2</sub>. X-ray diffraction (XRD) was employed to characterize changes to the <i>HA</i> structure as the dopants (Cu<sup>2+</sup>, Ag<sup>+</sup>) were incorporated into the materials structure. Energy-dispersive X-ray spectroscopy (EDS) determined confirmed the compositions and found that the Ca/P ratio was 1.63 for the control (<i>HA</i>) while <i>Ag-HA</i> and <i>Cu-HA</i> exhibited (X + Ca)/P ratios of 1.79 and 1.65, respectively. Antibacterial efficacies were evaluated against <i>E</i>. <i>coli</i> and <i>S</i>. <i>aureus</i>, as a function of surface area and incubation time. The more prominent antibacterial effects were observed with both <i>Ag-HA</i> and <i>Cu-HA</i> and the materials antibacterial influence was maintained with respect to time. Ion release studies of each <i>HA</i> composition (15, 30, and 45 days) showed that <i>Cu-HA</i> released significantly more Cu<sup>2+</sup> (36.1 ± 5.1 mg/L) than Ag<sup>+</sup> (2.9 ± 1.2 mg/L) from <i>Ag-HA</i>. Analysis of each composition incubated in simulated body fluid (SBF) exhibited surface depositions that are likely calcium phosphate (CaP). Cytocompatibility testing in MC 3T3 Osteoblasts showed slight reductions in cell viability when tested using MTT assay, however cell adhesion studies were positive for each composition.</p>","PeriodicalId":15269,"journal":{"name":"Journal of biomedical materials research. Part B, Applied biomaterials","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2022-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical materials research. Part B, Applied biomaterials","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.35151","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 3
Abstract
Hydroxyapatite (HA) powders were synthesized by the wet precipitation method in which two experimental compositions were synthesized (10 mol% Ag-HA and Cu-HA) where the CaNO3 content was partially substituted with AgNO3 and Cu(NO3)2. X-ray diffraction (XRD) was employed to characterize changes to the HA structure as the dopants (Cu2+, Ag+) were incorporated into the materials structure. Energy-dispersive X-ray spectroscopy (EDS) determined confirmed the compositions and found that the Ca/P ratio was 1.63 for the control (HA) while Ag-HA and Cu-HA exhibited (X + Ca)/P ratios of 1.79 and 1.65, respectively. Antibacterial efficacies were evaluated against E. coli and S. aureus, as a function of surface area and incubation time. The more prominent antibacterial effects were observed with both Ag-HA and Cu-HA and the materials antibacterial influence was maintained with respect to time. Ion release studies of each HA composition (15, 30, and 45 days) showed that Cu-HA released significantly more Cu2+ (36.1 ± 5.1 mg/L) than Ag+ (2.9 ± 1.2 mg/L) from Ag-HA. Analysis of each composition incubated in simulated body fluid (SBF) exhibited surface depositions that are likely calcium phosphate (CaP). Cytocompatibility testing in MC 3T3 Osteoblasts showed slight reductions in cell viability when tested using MTT assay, however cell adhesion studies were positive for each composition.
期刊介绍：
Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats:
• original research reports
• short research and development reports
• scientific reviews
• current concepts articles
• special reports
• editorials
Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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