Coding Variants of the FMO3 Gene Are Associated with the Risk of Chronic Kidney Disease: A Case-Control Study.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2022-10-01 DOI:10.52547/rbmb.11.3.430
Ismail Shorudi Dadi, Ramin Saravani, Tahereh Khalili, Saman Sargazi, Mahdi Majidpour, Mohammad Sarhadi, Shekoufeh Mirinejad, Sheida Shahraki, Ali Alidadi
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Abstract

Background: Chronic kidney disease (CKD) is a global health concern involving roughly one-tenth of developed countries' populations. The flavin-containing dimethylaniline monooxygenase 3 (FMO3) gene encodes an enzyme that catalyzes trimethylamine N-oxide (TMAO), a toxin in CKD sufferers. This preliminary study aims to evaluate the association between coding region variations of FMO3, rs2266782G/A (E158K), rs2266780A/G (E308G), and rs1736557G/A (V257M), and the susceptibility to CKD.

Methods: A total of 356 participants were enrolled, including 157 patients diagnosed with CKD and 199 age-matched healthy individuals. Genotyping of FMO3 gene variations was performed via PCR-RFLP and ARMS-PCR methods.

Results: Our findings revealed a significant association between rs2266780A/G and rs1736557G/A and CKD under different genetic models. Compared to the GGG haplotype of rs2266782/rs1736557/rs2266780, the GAG, GAA, AAG, and AAA haplotype combinations conferred an increased risk of CKD in our population. Interaction analysis revealed that some genotype combinations, including GA/AA/AA, AA/AA/AA, GA/AA/GA, and GG/AG/AA, dramatically increased CKD risk in the Iranian population. No correlation was found between FMO3 polymorphisms and CKD stages.

Discussion: These observations highlight the potential impact of coding variants of the FMO3 gene on the onset of CKD. Further investigations into expanded populations and diverse races are needed to confirm our findings.

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FMO3基因的编码变异与慢性肾脏疾病的风险相关:一项病例对照研究
背景:慢性肾脏疾病(CKD)是一个全球性的健康问题,约占发达国家人口的十分之一。含有黄素的二甲苯胺单加氧酶3 (FMO3)基因编码一种催化三甲胺n-氧化物(TMAO)的酶,TMAO是CKD患者体内的一种毒素。本初步研究旨在评估FMO3、rs2266782G/A (E158K)、rs2266780A/G (E308G)和rs1736557G/A (V257M)编码区变异与CKD易感性的关系。方法:共纳入356名参与者,包括157名诊断为CKD的患者和199名年龄匹配的健康个体。采用PCR-RFLP和ARMS-PCR方法对FMO3基因变异进行分型。结果:我们的研究结果揭示了rs2266780A/G和rs1736557G/ a与CKD在不同遗传模型下的显著相关性。与rs2266782/rs1736557/rs2266780的GGG单倍型相比,GAG、GAA、AAG和AAA单倍型组合在我们的人群中增加了CKD的风险。相互作用分析显示,GA/AA/AA、AA/AA/AA、GA/AA/GA、GG/AG/AA等基因型组合显著增加伊朗人群CKD风险。FMO3多态性与CKD分期无相关性。讨论:这些观察结果强调了FMO3基因编码变异对CKD发病的潜在影响。需要对扩大的人口和不同的种族进行进一步的调查来证实我们的发现。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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