Expression levels of miR-22, miR-30c, miR-145, and miR-519d and their possible associations with inflammatory markers among patients with coronary artery disease.

IF 0.5 Q4 CARDIAC & CARDIOVASCULAR SYSTEMS ARYA Atherosclerosis Pub Date : 2022-05-01 DOI:10.48305/arya.v18i0.2436

Saied Ghorbani, Seyed Hashem Sezavar, Farah Bokharaei-Salim, Angila Ataei-Pirkooh, Ahmad Tavakoli, Davod Javanmard, Javid Sadri-Nahand, Seyed Jalal Kiani, Hadi Ghaffari, Leila Beikzadeh, Latif Hamidpoor, Seyed Hamidreza Monavari

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引用次数: 1

Abstract

Background: Coronary artery disease (CAD) is a leading cause of death around the world. Micro-ribonucleic acid (miRNA) can be involved in forming of atherosclerotic plaques, inflammation, cholesterol metabolism, and other mechanisms involved in CAD development. This study aimed to evaluate the expression level of miR-22, miR-30c, miR-145, and miR-519d and their possible association with inflammatory markers among patients with CAD.

Methods: The expression level of miR-22, miR-30c, miR-145, miR-519d, interleukin 6 (IL-6), and transforming growth factor beta (TGF-β) was determined in peripheral blood mononuclear cells (PBMCs) from 46 patients with CAD and 39 healthy controls using real-time quantitative polymerase chain reaction (qPCR) assay.

Results: 53.8% (n = 21) and 52.2% (n = 24) of controls and cases were men, respectively; the mean age was 59.8 ± 7.4 and 57.0 ± 9.8 years, respectively. The miRNA expression pattern of each group showed significantly different expression profiles. In the CAD patients group, miR-22, miR-30c, and miR-145 were down-regulated compared to the control group. On the opposite, miR-519d was up-regulated in patients with CAD compared to the control group. Our results also showed that the expression levels of IL-6 and TGF-β were up-regulated among patients with CAD compared to the control group. In addition, the expression of miR-145 and miR-519d had a significantly negative and positive correlation with TGF-β and IL-6, respectively.

Conclusion: The change in expression levels of miR-22, miR-30c, miR-145, and miR-519d in PBMCs of patients with CAD could be considered as a potential biomarker for CAD.

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冠状动脉疾病患者中miR-22、miR-30c、miR-145和miR-519d的表达水平及其与炎症标志物的可能关联

背景:冠状动脉疾病(CAD)是世界范围内死亡的主要原因之一。微核糖核酸(miRNA)可能参与动脉粥样硬化斑块的形成、炎症、胆固醇代谢和其他与CAD发展有关的机制。本研究旨在评估CAD患者中miR-22、miR-30c、miR-145和miR-519d的表达水平及其与炎症标志物的可能关联。方法:采用实时定量聚合酶链反应(qPCR)法检测46例冠心病患者和39例健康对照者外周血单个核细胞(PBMCs)中miR-22、miR-30c、miR-145、miR-519d、白细胞介素6 (IL-6)和转化生长因子β (TGF-β)的表达水平。结果:男性占对照组的53.8% (n = 21)，占病例的52.2% (n = 24);平均年龄分别为59.8±7.4岁和57.0±9.8岁。各组miRNA表达谱差异有统计学意义。在CAD患者组中，miR-22、miR-30c和miR-145与对照组相比下调。相反，与对照组相比，CAD患者中miR-519d表达上调。我们的研究结果还显示，与对照组相比，冠心病患者IL-6和TGF-β的表达水平上调。此外，miR-145和miR-519d的表达分别与TGF-β和IL-6呈显著负相关和正相关。结论:CAD患者PBMCs中miR-22、miR-30c、miR-145和miR-519d表达水平的变化可被认为是CAD的潜在生物标志物。

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