Cathelicidin LL-37 promotes EMT, migration and metastasis of hepatocellular carcinoma cells in vitro and mouse model.

IF 3.3 3区生物学 Q3 CELL BIOLOGY Cell Adhesion & Migration Pub Date : 2023-12-01 DOI:10.1080/19336918.2023.2168231

Huidan Zhang, Xueli Yuan, Yaxin Yang, Yangke Wanyan, Liping Tao, Yuqing Chen

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引用次数: 2

Abstract

The effect of cathelicidin hCAP18/LL-37 in hepatocellular carcinoma (HCC) metastasis remains unclear. Here, we confirmed that LL-37 expression enhanced endothelial-mesenchymal transition (EMT), migration and invasion in HCC cells. And the HER2/EGFR-MAPK/ERK signal participated in the process above. More frequent lung metastases were observed in an LL-37-overexpressing hematogenous metastasis model. Interestingly, 1,25(OH)₂D₃ together with si-LL-37 significantly enhanced 1,25(OH)₂D₃-induced inhibition of migration and invasion in PLC/PRF-5 cells, and also enhanced reversion of the EMT process. Therefore, LL-37 is involved in HCC metastases, and may act as an important factor to attenuate the inhibitory activity of 1,25(OH)₂D₃ on HCC metastasis. Targeting hCAP18/LL-37 may offer a potential strategy to improve the anticancer activity of 1,25(OH)₂D₃ in HCC therapy.

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Cathelicidin LL-37在体外和小鼠模型中促进肝癌细胞的EMT、迁移和转移。

cathelicidin-hCAP18/LL-37在肝细胞癌（HCC）转移中的作用尚不清楚。在这里，我们证实LL-37的表达增强了HCC细胞中的内皮-间充质转化（EMT）、迁移和侵袭。HER2/EGFR-MAPK/ERK信号参与了上述过程。在LL-37-过表达血行转移模型中观察到更频繁的肺转移。有趣的是，1,25（OH）2D3与si-LL-37一起显著增强了1,25（羟基）2D3诱导的对PLC/PRF-5细胞迁移和侵袭的抑制，并增强了EMT过程的逆转。因此，LL-37参与HCC转移，并且可能作为减弱1，25（OH）2D3对HCC转移的抑制活性的重要因素。靶向hCAP18/LL-37可能提供一种在HCC治疗中提高1，25（OH）2D3抗癌活性的潜在策略。

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来源期刊

Cell Adhesion & Migration CELL BIOLOGY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

53 weeks

期刊介绍： Cell Adhesion & Migration is a multi-disciplinary, peer reviewed open access journal that focuses on the biological or pathological implications of cell-cell and cell-microenvironment interactions. The main focus of this journal is fundamental science. The journal strives to serve a broad readership by regularly publishing review articles covering specific disciplines within the field, and by publishing focused issues that provide an overview on specific topics of interest within the field. Cell Adhesion & Migration publishes relevant and timely original research, as well as authoritative overviews, commentaries, and perspectives, providing context for the work presented in Cell Adhesion & Migration and for key results published elsewhere. Original research papers may cover all topics important in the field of cell-cell and cell-matrix interactions. Cell Adhesion & Migration also publishes articles related to cell biomechanics, biomaterial, and development of related imaging technologies.