Fabrication and characterization of super-hydrophilic poly (ε-caprolactone)/hydroxypropyl methylcellulose (HPMC) based composite electrospun membranes for tissue engineering applications.

IF 4.4 3区医学 Q2 ENGINEERING, BIOMEDICAL Progress in Biomaterials Pub Date : 2023-03-01 DOI:10.1007/s40204-022-00205-7

B Sowmya, P K Panda

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Abstract

Tissue engineering (TE) employs scaffolds as a structural support for initially seeding of cells followed by development of new tissues. Electrospun scaffolds generally function as a template of native extracellular matrix (ECM). The chemical composition of the scaffold and its surface morphology strongly influence the interaction between various cell types and materials. In this work, PCL and PCL/HPMC-based composite membranes with varying concentrations of HPMC (20-30% by weight) were fabricated using electrospinning technique. The membranes were evaluated for their surface, physio-chemical and biological properties. It was observed probably for the first time that blending of HPMC with PCL produced super-hydrophilic scaffolds. DSC studies confirmed the semi- crystalline nature of HPMC. PCL/HPMC composite scaffolds are found biocompatible from cytotoxicity assay. From the cell culture studies (apoptosis), PCL/HPMC composite scaffolds did not inhibit the adhesion of L929 cells due to their super-hydrophilic nature. The cell adhesion and spreading varied with HPMC concentration. PCL/HPMC (70/30) membranes showed highest cell adhesion among others due to its porous structure.

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超亲水性聚(ε-己内酯)/羟丙基甲基纤维素(HPMC)复合静电纺丝膜的制备与表征

组织工程(TE)采用支架作为最初的细胞播种的结构支持，随后是新组织的发展。电纺丝支架通常作为细胞外基质(ECM)的模板。支架的化学成分及其表面形态强烈地影响着各种细胞类型和材料之间的相互作用。本研究采用静电纺丝技术制备了PCL和PCL/HPMC基复合膜，HPMC浓度为20-30%。对膜的表面、理化和生物学性能进行了评价。这可能是首次观察到HPMC与PCL共混产生超亲水性支架。DSC研究证实了HPMC的半结晶性质。细胞毒性实验表明，PCL/HPMC复合支架具有良好的生物相容性。从细胞培养研究(凋亡)来看，PCL/HPMC复合支架由于其超亲水性没有抑制L929细胞的粘附。细胞的粘附和扩散随HPMC浓度的变化而变化。PCL/HPMC(70/30)膜由于其多孔结构而具有最高的细胞粘附性。

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来源期刊

Progress in Biomaterials MATERIALS SCIENCE, BIOMATERIALS-

CiteScore

9.60

自引率

4.10%

发文量

期刊介绍： Progress in Biomaterials is a multidisciplinary, English-language publication of original contributions and reviews concerning studies of the preparation, performance and evaluation of biomaterials; the chemical, physical, biological and mechanical behavior of materials both in vitro and in vivo in areas such as tissue engineering and regenerative medicine, drug delivery and implants where biomaterials play a significant role. Including all areas of: design; preparation; performance and evaluation of nano- and biomaterials in tissue engineering; drug delivery systems; regenerative medicine; implantable medical devices; interaction of cells/stem cells on biomaterials and related applications.