Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats.

IF 2.8 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Molecular Oral Microbiology Pub Date : 2023-04-01 DOI:10.1111/omi.12402

Pedro Henrique Felix Silva, Luiz Fernando Ferreira Oliveira, Renata Silva Cardoso, Sandro Isaias Santana, Renato Correa Casarin, Edilson Ervolino, Sergio Luiz Salvador, Daniela Bazan Palioto, Flávia Aparecida Chaves Furlaneto, Michel Reis Messora

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引用次数: 1

Abstract

Aim: The aim of this study was to evaluate the effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis (EP) in rats.

Methods: Thirty-two rats were divided into four groups: control, C-HD100 (B. bacteriovorus), EP, and EP-HD100. On day 0, EP was induced by the placement of cotton ligatures around the mandibular first molars (MFMs) in the EP and EP-HD100 groups. In the C-HD100 and EP-HD100 groups, suspensions containing 1 × 10⁹ PUF/ml of B. bacteriovorus HD100 were topically administered to the subgingival region of MFMs on days 0, 3, and 7. Animals were euthanized on day 14. Morphometrics analyses were performed in hemimandibles. The levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1, IL-10, IL-1β, transforming growth factor beta (TGF-β), macrophage colony-stimulating factor (M-CSF) and regulated on activation and normal T cell expressed and secreted (RANTES) were determined by enzymatic immunoassays in gingival tissues. Beta defensin (BD)-1, BD-2, and BD-3, Toll-like receptors (TLR)-2 and TLR-4, and a cluster of differentiation (CD)-4, CD-8 and CD-57 were analyzed by immunohistochemistry in hemimandibles. Data were statistically analyzed.

Results: The EP group showed greater alveolar bone loss than EP-HD100 (p < .05). The EP-HD100 group showed higher levels of MCP-1, RANTES, IL-10, and TGF-β, lower levels of TNF-α than the EP group (p < .05). No differences were observed in IL-1β, IL-6, and M-CSF levels between EP and EP-HD100 groups. The C-HD100 group had higher IL-6, TNF-α, RANTES, and MCP-1 levels than the control group (p < .05). Regarding BD, the EP-HD100 group showed a larger immunolabeling pattern for BD-1, BD-2, and BD-3 than the EP group (p < .05). No significant differences in the immunolabeling pattern were observed for TLR-2, TLR-4, CD-4, CD-8, and CD-57 between EP and EP-HD100 groups.

Conclusion: The topical use of B. bacteriovorus HD100 reduces alveolar bone loss, increases expression of BD, and modulates the cytokines levels on periodontal tissues in rats with EP.

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乳杆菌弧菌HD100对实验性大鼠牙周炎的影响。

目的:探讨产蛋弧菌HD100对实验性牙周炎(EP)的治疗作用。方法:32只大鼠随机分为对照组、C-HD100组、EP组和EP- hd100组。第0天，EP组和EP- hd100组在下颌第一磨牙(MFMs)周围放置棉花结扎诱导EP。在C-HD100和EP-HD100组中，含1 × 109 PUF/ml的B. bacteriovorus HD100的混悬液于0、3和7天局部给予MFMs龈下区域。在第14天对动物实施安乐死。对半下颌骨进行形态计量学分析。采用酶免疫法检测牙龈组织中肿瘤坏死因子α (TNF-α)、白细胞介素(IL)-6、单核细胞趋化蛋白(MCP)-1、IL-10、IL-1β、转化生长因子β (TGF-β)、巨噬细胞集落刺激因子(M-CSF)、活化调控及正常T细胞表达和分泌(RANTES)水平。采用免疫组化方法分析了半下丘脑中β -防御素(BD)-1、BD-2、BD-3、toll样受体(TLR)-2、TLR-4以及一簇分化(CD)-4、CD-8、CD-57。对数据进行统计学分析。结果:EP组牙槽骨缺损明显大于EP-HD100组(p)。结论:外用B. bacteriovorus HD100可减少EP大鼠牙槽骨缺损，增加BD表达，调节牙周组织细胞因子水平。

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来源期刊

Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY

CiteScore

6.50

自引率

5.40%

发文量

审稿时长

>12 weeks

期刊介绍： Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.