SlZF3 regulates tomato plant height by directly repressing SlGA20ox4 in the gibberellic acid biosynthesis pathway.

IF 8.7 1区农林科学 Q1 Agricultural and Biological Sciences Horticulture Research Pub Date : 2023-04-01 DOI:10.1093/hr/uhad025

Jinying Luo, Yunfei Tang, Zhuannan Chu, Yuxin Peng, Jiawei Chen, Huiyang Yu, Chunmei Shi, Jahanzeb Jafar, Rong Chen, Yaping Tang, Yongen Lu, Zhibiao Ye, Ying Li, Bo Ouyang

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引用次数: 1

Abstract

Plant height is an important target trait for crop genetic improvement. Our previous work has identified a salt-tolerant C2H2 zinc finger, SlZF3, and its overexpression lines also showed a semi-dwarf phenotype, but the molecular mechanism remains to be elucidated. Here, we characterized the dwarf phenotype in detail. The dwarfism is caused by a decrease in stem internode cell elongation and deficiency of bioactive gibberellic acids (GAs), and can be rescued by exogenous GA3 treatment. Gene expression assays detected reduced expression of genes in the GA biosynthesis pathway of the overexpression lines, including SlGA20ox4. Several protein-DNA interaction methods confirmed that SlZF3 can directly bind to the SlGA20ox4 promoter and inhibit its expression, and the interaction can also occur for SlKS and SlKO. Overexpression of SlGA20ox4 in the SlZF3-overexpressing line can recover the dwarf phenotype. Therefore, SlZF3 regulates plant height by directly repressing genes in the tomato GA biosynthesis pathway.

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SlZF3通过直接抑制赤霉素合成途径中的SlGA20ox4调控番茄株高。

株高是作物遗传改良的重要目标性状。我们之前的工作已经鉴定出耐盐C2H2锌指SlZF3，其过表达系也表现出半矮化表型，但分子机制仍有待阐明。在这里，我们详细描述了矮化表型。矮化是由茎节间细胞伸长减少和生物活性赤霉素(GAs)缺乏引起的，可以通过外源GA3处理来挽救。基因表达分析发现，包括SlGA20ox4在内的过表达系的GA生物合成途径基因表达减少。多种蛋白- dna相互作用方法证实，SlZF3可以直接结合SlGA20ox4启动子并抑制其表达，SlKS和SlKO也可以发生相互作用。SlGA20ox4在slzf3过表达系中过表达可恢复矮化表型。因此，SlZF3通过直接抑制番茄GA生物合成途径中的基因调控株高。

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来源期刊

Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

11.20

自引率

6.90%

发文量

367

审稿时长

20 weeks

期刊介绍： Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.