Ji Hyung Kim, Jee In Yoo, Hyun Woo Ma, I Seul Park, Mijeong Son, Yoojin Shin, Ki Beom Kim, Seung Won Kim, Si Jae Park, Jihye Park
{"title":"Anti-inflammatory properties of butyrate-producing atypical Escherichia coli in a murine colitis model.","authors":"Ji Hyung Kim, Jee In Yoo, Hyun Woo Ma, I Seul Park, Mijeong Son, Yoojin Shin, Ki Beom Kim, Seung Won Kim, Si Jae Park, Jihye Park","doi":"10.5217/ir.2022.00112","DOIUrl":null,"url":null,"abstract":"This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 1 ing a 2-plasmid system capable of introducing foreign genes and overexpressing genes for gene expression in the butyric acid metabolic cycle (Supplementary Fig. 1, Supplementary Table 1). Animals were randomly divided into 4 groups; control+phosphate buffered saline (PBS) (n = 3), dextran sulfate sodium (DSS)+PBS (n = 4), DSS+atEc (n = 4), and DSS+butyrate atEc groups (n = 4). Male C57BL/6 mice (8-week-old) were treated with 1 × 10 bacteria in 0.2 mL PBS daily by oral gavage before and after the induction of DSS colitis (from day 0 to day 12) (Fig. 1A). DSS (2.5% [w/v]; MP Biomedicals, Solon, OH, USA) was added to their drinking water from day 3 of gavage and continued for day 9, and water was changed to DSS-free water before euthanization. All mice were sacrificed 12 days after the onset of the study. Mice were monitored for survival, body weight loss, disease activity index, and colon length. Parts of the distal colon were cut into 2 to 3 pieces for periodic acid-Schiff staining and histologic scoring was obtained. All animal testing complied with all applicable Korean laws and was approved by the Institutional Animal Care and Use Committee of Yonsei University Severance Hospital (Seoul, Korea) (IACUC No. 2019-0241). The experiments complied with the approved IACUC guidelines. All results are expressed as standard error of the mean. GraphPad Software (La Jolla, CA, USA) was used for all analyses. The significance of differences between conditions was assessed using one-way or two-way analysis of variance followed by Holm-Sidak or Tukey’s multiple comparison test, respectively. pISSN 1598-9100 • eISSN 2288-1956 https://doi.org/10.5217/ir.2022.00112 Intest Res, Published online November 14, 2022","PeriodicalId":14481,"journal":{"name":"Intestinal Research","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0c/1b/ir-2022-00112.PMC10169521.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Intestinal Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5217/ir.2022.00112","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 1 ing a 2-plasmid system capable of introducing foreign genes and overexpressing genes for gene expression in the butyric acid metabolic cycle (Supplementary Fig. 1, Supplementary Table 1). Animals were randomly divided into 4 groups; control+phosphate buffered saline (PBS) (n = 3), dextran sulfate sodium (DSS)+PBS (n = 4), DSS+atEc (n = 4), and DSS+butyrate atEc groups (n = 4). Male C57BL/6 mice (8-week-old) were treated with 1 × 10 bacteria in 0.2 mL PBS daily by oral gavage before and after the induction of DSS colitis (from day 0 to day 12) (Fig. 1A). DSS (2.5% [w/v]; MP Biomedicals, Solon, OH, USA) was added to their drinking water from day 3 of gavage and continued for day 9, and water was changed to DSS-free water before euthanization. All mice were sacrificed 12 days after the onset of the study. Mice were monitored for survival, body weight loss, disease activity index, and colon length. Parts of the distal colon were cut into 2 to 3 pieces for periodic acid-Schiff staining and histologic scoring was obtained. All animal testing complied with all applicable Korean laws and was approved by the Institutional Animal Care and Use Committee of Yonsei University Severance Hospital (Seoul, Korea) (IACUC No. 2019-0241). The experiments complied with the approved IACUC guidelines. All results are expressed as standard error of the mean. GraphPad Software (La Jolla, CA, USA) was used for all analyses. The significance of differences between conditions was assessed using one-way or two-way analysis of variance followed by Holm-Sidak or Tukey’s multiple comparison test, respectively. pISSN 1598-9100 • eISSN 2288-1956 https://doi.org/10.5217/ir.2022.00112 Intest Res, Published online November 14, 2022
期刊介绍:
Intestinal Research (Intest Res) is the joint official publication of the Asian Organization for Crohn''s and Colitis (AOCC), Chinese Society of IBD (CSIBD), Japanese Society for IBD (JSIBD), Korean Association for the Study of Intestinal Diseases (KASID), Taiwan Society of IBD (TSIBD) and Colitis Crohn''s Foundation (India) (CCF, india). The aim of the Journal is to provide broad and in-depth analysis of intestinal diseases, especially inflammatory bowel disease, which shows increasing tendency and significance. As a Journal specialized in clinical and translational research in gastroenterology, it encompasses multiple aspects of diseases originated from the small and large intestines. The Journal also seeks to propagate and exchange useful innovations, both in ideas and in practice, within the research community. As a mode of scholarly communication, it encourages scientific investigation through the rigorous peer-review system and constitutes a qualified and continual platform for sharing studies of researchers and practitioners. Specifically, the Journal presents up-to-date coverage of medical researches on the physiology, epidemiology, pathophysiology, clinical presentations, and therapeutic interventions of the intestinal diseases. General topics of interest include inflammatory bowel disease, colon and small intestine cancer or polyp, endoscopy, irritable bowel syndrome and other motility disorders, infectious enterocolitis, intestinal tuberculosis, and so forth. The Journal publishes diverse types of academic materials such as editorials, clinical and basic reviews, original articles, case reports, letters to the editor, brief communications, perspective, statement or commentary, and images that are useful to clinicians and researchers.