Anti-inflammatory properties of butyrate-producing atypical Escherichia coli in a murine colitis model.

IF 3.4 Q2 GASTROENTEROLOGY & HEPATOLOGY Intestinal Research Pub Date : 2023-04-01 DOI:10.5217/ir.2022.00112
Ji Hyung Kim, Jee In Yoo, Hyun Woo Ma, I Seul Park, Mijeong Son, Yoojin Shin, Ki Beom Kim, Seung Won Kim, Si Jae Park, Jihye Park
{"title":"Anti-inflammatory properties of butyrate-producing atypical Escherichia coli in a murine colitis model.","authors":"Ji Hyung Kim, Jee In Yoo, Hyun Woo Ma, I Seul Park, Mijeong Son, Yoojin Shin, Ki Beom Kim, Seung Won Kim, Si Jae Park, Jihye Park","doi":"10.5217/ir.2022.00112","DOIUrl":null,"url":null,"abstract":"This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 1 ing a 2-plasmid system capable of introducing foreign genes and overexpressing genes for gene expression in the butyric acid metabolic cycle (Supplementary Fig. 1, Supplementary Table 1). Animals were randomly divided into 4 groups; control+phosphate buffered saline (PBS) (n = 3), dextran sulfate sodium (DSS)+PBS (n = 4), DSS+atEc (n = 4), and DSS+butyrate atEc groups (n = 4). Male C57BL/6 mice (8-week-old) were treated with 1 × 10 bacteria in 0.2 mL PBS daily by oral gavage before and after the induction of DSS colitis (from day 0 to day 12) (Fig. 1A). DSS (2.5% [w/v]; MP Biomedicals, Solon, OH, USA) was added to their drinking water from day 3 of gavage and continued for day 9, and water was changed to DSS-free water before euthanization. All mice were sacrificed 12 days after the onset of the study. Mice were monitored for survival, body weight loss, disease activity index, and colon length. Parts of the distal colon were cut into 2 to 3 pieces for periodic acid-Schiff staining and histologic scoring was obtained. All animal testing complied with all applicable Korean laws and was approved by the Institutional Animal Care and Use Committee of Yonsei University Severance Hospital (Seoul, Korea) (IACUC No. 2019-0241). The experiments complied with the approved IACUC guidelines. All results are expressed as standard error of the mean. GraphPad Software (La Jolla, CA, USA) was used for all analyses. The significance of differences between conditions was assessed using one-way or two-way analysis of variance followed by Holm-Sidak or Tukey’s multiple comparison test, respectively. pISSN 1598-9100 • eISSN 2288-1956 https://doi.org/10.5217/ir.2022.00112 Intest Res, Published online November 14, 2022","PeriodicalId":14481,"journal":{"name":"Intestinal Research","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0c/1b/ir-2022-00112.PMC10169521.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Intestinal Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5217/ir.2022.00112","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 1 ing a 2-plasmid system capable of introducing foreign genes and overexpressing genes for gene expression in the butyric acid metabolic cycle (Supplementary Fig. 1, Supplementary Table 1). Animals were randomly divided into 4 groups; control+phosphate buffered saline (PBS) (n = 3), dextran sulfate sodium (DSS)+PBS (n = 4), DSS+atEc (n = 4), and DSS+butyrate atEc groups (n = 4). Male C57BL/6 mice (8-week-old) were treated with 1 × 10 bacteria in 0.2 mL PBS daily by oral gavage before and after the induction of DSS colitis (from day 0 to day 12) (Fig. 1A). DSS (2.5% [w/v]; MP Biomedicals, Solon, OH, USA) was added to their drinking water from day 3 of gavage and continued for day 9, and water was changed to DSS-free water before euthanization. All mice were sacrificed 12 days after the onset of the study. Mice were monitored for survival, body weight loss, disease activity index, and colon length. Parts of the distal colon were cut into 2 to 3 pieces for periodic acid-Schiff staining and histologic scoring was obtained. All animal testing complied with all applicable Korean laws and was approved by the Institutional Animal Care and Use Committee of Yonsei University Severance Hospital (Seoul, Korea) (IACUC No. 2019-0241). The experiments complied with the approved IACUC guidelines. All results are expressed as standard error of the mean. GraphPad Software (La Jolla, CA, USA) was used for all analyses. The significance of differences between conditions was assessed using one-way or two-way analysis of variance followed by Holm-Sidak or Tukey’s multiple comparison test, respectively. pISSN 1598-9100 • eISSN 2288-1956 https://doi.org/10.5217/ir.2022.00112 Intest Res, Published online November 14, 2022

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
小鼠结肠炎模型中产生丁酸酯的非典型大肠杆菌的抗炎特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Intestinal Research
Intestinal Research GASTROENTEROLOGY & HEPATOLOGY-
CiteScore
7.40
自引率
10.20%
发文量
69
审稿时长
38 weeks
期刊介绍: Intestinal Research (Intest Res) is the joint official publication of the Asian Organization for Crohn''s and Colitis (AOCC), Chinese Society of IBD (CSIBD), Japanese Society for IBD (JSIBD), Korean Association for the Study of Intestinal Diseases (KASID), Taiwan Society of IBD (TSIBD) and Colitis Crohn''s Foundation (India) (CCF, india). The aim of the Journal is to provide broad and in-depth analysis of intestinal diseases, especially inflammatory bowel disease, which shows increasing tendency and significance. As a Journal specialized in clinical and translational research in gastroenterology, it encompasses multiple aspects of diseases originated from the small and large intestines. The Journal also seeks to propagate and exchange useful innovations, both in ideas and in practice, within the research community. As a mode of scholarly communication, it encourages scientific investigation through the rigorous peer-review system and constitutes a qualified and continual platform for sharing studies of researchers and practitioners. Specifically, the Journal presents up-to-date coverage of medical researches on the physiology, epidemiology, pathophysiology, clinical presentations, and therapeutic interventions of the intestinal diseases. General topics of interest include inflammatory bowel disease, colon and small intestine cancer or polyp, endoscopy, irritable bowel syndrome and other motility disorders, infectious enterocolitis, intestinal tuberculosis, and so forth. The Journal publishes diverse types of academic materials such as editorials, clinical and basic reviews, original articles, case reports, letters to the editor, brief communications, perspective, statement or commentary, and images that are useful to clinicians and researchers.
期刊最新文献
Exploring the link between celiac disease and obesity: a potential role of gut microbiome. Optimizing 5-aminosalicylate for moderate ulcerative colitis: expert recommendations from the Asia-Pacific, Middle East, and Africa Inflammatory Bowel Disease Coalition. Patient preferences for advanced therapies in ulcerative colitis using conjoint analysis. The evolving understanding of histology as an endpoint in ulcerative colitis. Ulcerative colitis-associated neoplasms often harbor poor prognostic histologic components with low detection by biopsy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1