Isolation and identification of an arbuscular mycorrhizal fungus specifically associated with mycoheterotrophic seedlings of Gentiana zollingeri (Gentianaceae).

IF 1.5 4区 生物学 Q4 MYCOLOGY Mycoscience Pub Date : 2023-01-01 DOI:10.47371/mycosci.2023.01.001
Ryota Kusakabe, Masahide Yamato
{"title":"Isolation and identification of an arbuscular mycorrhizal fungus specifically associated with mycoheterotrophic seedlings of <i>Gentiana zollingeri</i> (<i>Gentianaceae</i>).","authors":"Ryota Kusakabe,&nbsp;Masahide Yamato","doi":"10.47371/mycosci.2023.01.001","DOIUrl":null,"url":null,"abstract":"<p><p><i>Gentiana zollingeri</i> (<i>Gentianaceae</i>) is an initial mycoheterotrophic plant that depends on a specific group of arbuscular mycorrhizal (AM) fungi for carbon source during underground growth after seed germination. In this study, a mycorrhizal fungus dominant in mycoheterotrophic seedlings of <i>G. zollingeri</i> was successfully isolated from a soil core collected from a point close to a flowering <i>G. zollingeri</i>. The AM fungal isolate was identified as conspecific or closely related to <i>Dominikia aurea</i> (<i>Glomeraceae</i>) by spore morphology and molecular phylogeny. Basic Local Alignment Search Tool (BLAST) searches against the Maarj<i>AM</i> database showed that the nuclear small subunit ribosomal DNA sequences of the isolate matched the AM fungal sequences obtained from a wide range of plants in various ecosystems, including several mycoheterotrophs. Thus, it is suggested that the AM fungal isolate is one of the cheating susceptible AM fungi. Furthermore, the sequences corresponded to those of a group of AM fungi dominantly detected in Japanese temperate forests. Accordingly, there is a possibility that mycoheterotrophic plants, including seedlings of <i>G. zollingeri</i>, may target AM fungi with a wide host range and ubiquitous distribution.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d2/43/MYC-64-055.PMC10165169.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycoscience","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.47371/mycosci.2023.01.001","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 1

Abstract

Gentiana zollingeri (Gentianaceae) is an initial mycoheterotrophic plant that depends on a specific group of arbuscular mycorrhizal (AM) fungi for carbon source during underground growth after seed germination. In this study, a mycorrhizal fungus dominant in mycoheterotrophic seedlings of G. zollingeri was successfully isolated from a soil core collected from a point close to a flowering G. zollingeri. The AM fungal isolate was identified as conspecific or closely related to Dominikia aurea (Glomeraceae) by spore morphology and molecular phylogeny. Basic Local Alignment Search Tool (BLAST) searches against the MaarjAM database showed that the nuclear small subunit ribosomal DNA sequences of the isolate matched the AM fungal sequences obtained from a wide range of plants in various ecosystems, including several mycoheterotrophs. Thus, it is suggested that the AM fungal isolate is one of the cheating susceptible AM fungi. Furthermore, the sequences corresponded to those of a group of AM fungi dominantly detected in Japanese temperate forests. Accordingly, there is a possibility that mycoheterotrophic plants, including seedlings of G. zollingeri, may target AM fungi with a wide host range and ubiquitous distribution.

Abstract Image

Abstract Image

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
一株与龙胆异养真菌相关的丛枝菌根真菌的分离与鉴定。
龙胆(Gentiana zollingeri,龙胆科)是一种初始的异养真菌植物,种子萌发后在地下生长过程中依赖于一类特定的丛枝菌根真菌(AM)提供碳源。在本研究中,我们成功地从开花的佐林芝附近的土壤核中分离出一种在异养真菌幼苗中占优势的菌根真菌。经孢子形态和分子系统发育鉴定,AM真菌分离物与鹅毛苣属真菌(Glomeraceae)同源或密切相关。利用BLAST工具对MaarjAM数据库进行检索,结果显示分离物的核小亚基核糖体DNA序列与从多种生态系统植物中获得的AM真菌序列相匹配,包括几种异源真菌。因此,AM真菌分离物可能是AM真菌的欺骗易感菌之一。此外,这些序列与在日本温带森林中发现的一组AM真菌的序列一致。因此,包括G. zollingeri幼苗在内的真菌异养植物可能针对寄主范围广、分布普遍的AM真菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Mycoscience
Mycoscience MYCOLOGY-
CiteScore
3.00
自引率
7.10%
发文量
32
审稿时长
3 months
期刊介绍: Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.
期刊最新文献
Gene expression analysis for stem browning in the mushroom Lentinula edodes Morphological responses of two Aspergillus oryzae strains to various metal ions at different concentrations Leucoagaricus karjaticus (Agaricaceae), a new species from Maharashtra, India Henon bamboo flowering recorded first time in 120 years revealed how Aciculosporium take affects the floral organs of the host Phylogenetic classification and physiological and ecological traits of Metarhizium spp.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1