Isolation and identification of an arbuscular mycorrhizal fungus specifically associated with mycoheterotrophic seedlings of Gentiana zollingeri (Gentianaceae).

IF 1.5 4区 生物学 Q4 MYCOLOGY Mycoscience Pub Date : 2023-01-01 DOI:10.47371/mycosci.2023.01.001
Ryota Kusakabe, Masahide Yamato
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引用次数: 1

Abstract

Gentiana zollingeri (Gentianaceae) is an initial mycoheterotrophic plant that depends on a specific group of arbuscular mycorrhizal (AM) fungi for carbon source during underground growth after seed germination. In this study, a mycorrhizal fungus dominant in mycoheterotrophic seedlings of G. zollingeri was successfully isolated from a soil core collected from a point close to a flowering G. zollingeri. The AM fungal isolate was identified as conspecific or closely related to Dominikia aurea (Glomeraceae) by spore morphology and molecular phylogeny. Basic Local Alignment Search Tool (BLAST) searches against the MaarjAM database showed that the nuclear small subunit ribosomal DNA sequences of the isolate matched the AM fungal sequences obtained from a wide range of plants in various ecosystems, including several mycoheterotrophs. Thus, it is suggested that the AM fungal isolate is one of the cheating susceptible AM fungi. Furthermore, the sequences corresponded to those of a group of AM fungi dominantly detected in Japanese temperate forests. Accordingly, there is a possibility that mycoheterotrophic plants, including seedlings of G. zollingeri, may target AM fungi with a wide host range and ubiquitous distribution.

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一株与龙胆异养真菌相关的丛枝菌根真菌的分离与鉴定。
龙胆(Gentiana zollingeri,龙胆科)是一种初始的异养真菌植物,种子萌发后在地下生长过程中依赖于一类特定的丛枝菌根真菌(AM)提供碳源。在本研究中,我们成功地从开花的佐林芝附近的土壤核中分离出一种在异养真菌幼苗中占优势的菌根真菌。经孢子形态和分子系统发育鉴定,AM真菌分离物与鹅毛苣属真菌(Glomeraceae)同源或密切相关。利用BLAST工具对MaarjAM数据库进行检索,结果显示分离物的核小亚基核糖体DNA序列与从多种生态系统植物中获得的AM真菌序列相匹配,包括几种异源真菌。因此,AM真菌分离物可能是AM真菌的欺骗易感菌之一。此外,这些序列与在日本温带森林中发现的一组AM真菌的序列一致。因此,包括G. zollingeri幼苗在内的真菌异养植物可能针对寄主范围广、分布普遍的AM真菌。
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来源期刊
Mycoscience
Mycoscience MYCOLOGY-
CiteScore
3.00
自引率
7.10%
发文量
32
审稿时长
3 months
期刊介绍: Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.
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