Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma.

IF 3.5 3区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL PPAR Research Pub Date : 2023-01-01 DOI:10.1155/2023/6797694
Chao Lu, Shenglin Gao, Li Zhang, Xiaokai Shi, Yin Chen, Shuzhang Wei, Li Zuo, Lifeng Zhang
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Abstract

Background: The Nuclear protein 1 gene was first discovered in acute pancreatitis and functions as an oncogene in cancer progression and drug resistance. However, the role of Nuclear protein 1 in bladder transitional cell carcinoma (BTCC) is still unclear.

Methods: The Cancer Genome Atlas database and immunohistochemical analysis were adopted to evaluate Nuclear protein 1 expression in BTCC. We applied lentivirus-mediated small-interfering RNA to down-regulate the expression of Nuclear protein 1 in BTCC cell lines. We further performed an Affymetrix microarray and Gene Set Enrichment Analysis (GSEA) to assess the genes and signaling pathways related to Nuclear protein 1.

Results: We found that Nuclear protein 1 expression was up-regulated in BTCC and positively related to the degree of BTCC malignancy. Compared with Caucasian patients with BTCC, Nuclear protein 1 expression was attenuated in Asian patients. The Affymetrix microarray showed that lipopolysaccharide was the upstream regulatory factor of Nuclear protein 1 in BTCC. The GSEA indicated that Nuclear protein 1 expression was associated with signaling pathways in cancer, peroxisome proliferator-activated receptor (PPAR) pathways, and RNA degradation. The expression of Nuclear protein 1 was negatively correlated with PPARG (R = -0.290, P < 0.001), but not with PPARA (R = 0.047, P = 0.344) and PPARD (R = -0.055, P = 0.260).

Conclusions: The study findings indicate that Nuclear protein 1 is positively associated with the malignancy degree of BTCC and that Nuclear protein 1 expression is negatively correlated with PPARG.

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核蛋白1在膀胱移行细胞癌中的表达与PPARG相关
背景:核蛋白1基因首次在急性胰腺炎中被发现,并在肿瘤进展和耐药过程中作为致癌基因发挥作用。然而,核蛋白1在膀胱移行细胞癌(BTCC)中的作用尚不清楚。方法:采用肿瘤基因组图谱数据库和免疫组化分析方法检测BTCC中核蛋白1的表达。应用慢病毒介导的小干扰RNA下调BTCC细胞株核蛋白1的表达。我们进一步使用Affymetrix微阵列和基因集富集分析(GSEA)来评估与核蛋白1相关的基因和信号通路。结果:我们发现核蛋白1在BTCC中表达上调,且与BTCC恶性程度呈正相关。与白种人BTCC患者相比,亚洲患者的核蛋白1表达减弱。Affymetrix微阵列检测结果显示,脂多糖是BTCC中核蛋白1的上游调控因子。GSEA表明,核蛋白1的表达与肿瘤信号通路、过氧化物酶体增殖物激活受体(PPAR)通路和RNA降解有关。核蛋白1的表达与PPARG呈负相关(R = -0.290, P < 0.001),与PPARA (R = 0.047, P = 0.344)、PPARD (R = -0.055, P = 0.260)无显著相关性。结论:研究结果表明,核蛋白1与BTCC恶性程度呈正相关,而核蛋白1的表达与PPARG呈负相关。
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来源期刊
PPAR Research
PPAR Research MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.20
自引率
3.40%
发文量
17
审稿时长
12 months
期刊介绍: PPAR Research is a peer-reviewed, Open Access journal that publishes original research and review articles on advances in basic research focusing on mechanisms involved in the activation of peroxisome proliferator-activated receptors (PPARs), as well as their role in the regulation of cellular differentiation, development, energy homeostasis and metabolic function. The journal also welcomes preclinical and clinical trials of drugs that can modulate PPAR activity, with a view to treating chronic diseases and disorders such as dyslipidemia, diabetes, adipocyte differentiation, inflammation, cancer, lung diseases, neurodegenerative disorders, and obesity.
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