Optogenetic Methods in Plant Biology.

IF 21.3 1区 生物学 Q1 PLANT SCIENCES Annual review of plant biology Pub Date : 2023-05-22 DOI:10.1146/annurev-arplant-071122-094840
Kai R Konrad, Shiqiang Gao, Matias D Zurbriggen, Georg Nagel
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引用次数: 2

Abstract

Optogenetics is a technique employing natural or genetically engineered photoreceptors in transgene organisms to manipulate biological activities with light. Light can be turned on or off, and adjusting its intensity and duration allows optogenetic fine-tuning of cellular processes in a noninvasive and spatiotemporally resolved manner. Since the introduction of Channelrhodopsin-2 and phytochrome-based switches nearly 20 years ago, optogenetic tools have been applied in a variety of model organisms with enormous success, but rarely in plants. For a long time, the dependence of plant growth on light and the absence of retinal, the rhodopsin chromophore, prevented the establishment of plant optogenetics until recent progress overcame these difficulties. We summarize the recent results of work in the field to control plant growth and cellular motion via green light-gated ion channels and present successful applications to light-control gene expression with single or combined photoswitches in plants. Furthermore, we highlight the technical requirements and options for future plant optogenetic research.

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植物生物学中的光遗传学方法。
光遗传学是一种利用转基因生物中的天然或基因工程光感受器来操纵光的生物活动的技术。光可以打开或关闭,并且调节其强度和持续时间允许以非侵入性和时空分辨的方式对细胞过程进行光遗传学微调。自近20年前引入通道视紫红质-2和基于光敏色素的开关以来,光遗传学工具已经在各种模式生物中获得了巨大的成功,但在植物中却很少应用。长期以来,植物生长对光的依赖和视紫红质发色团视网膜的缺失阻碍了植物光遗传学的建立,直到最近的进展克服了这些困难。本文综述了近年来通过绿光门控离子通道控制植物生长和细胞运动的研究成果,并介绍了利用单个或组合光开关在植物光控基因表达方面的成功应用。此外,我们还强调了未来植物光遗传研究的技术要求和选择。
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来源期刊
Annual review of plant biology
Annual review of plant biology 生物-植物科学
CiteScore
40.40
自引率
0.40%
发文量
29
期刊介绍: The Annual Review of Plant Biology is a peer-reviewed scientific journal published by Annual Reviews. It has been in publication since 1950 and covers significant developments in the field of plant biology, including biochemistry and biosynthesis, genetics, genomics and molecular biology, cell differentiation, tissue, organ and whole plant events, acclimation and adaptation, and methods and model organisms. The current volume of this journal has been converted from gated to open access through Annual Reviews' Subscribe to Open program, with all articles published under a CC BY license.
期刊最新文献
Adaptation and the Geographic Spread of Crop Species. Environmental Control of Hypocotyl Elongation. Plant Cryopreservation: Principles, Applications, and Challenges of Banking Plant Diversity at Ultralow Temperatures. Structure and Function of Auxin Transporters. Structural and Evolutionary Aspects of Plant Endocytosis.
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