A newly developed solution for the preservation of short-chain fatty acids, bile acids, and microbiota in fecal specimens.

IF 2 4区 医学 Q3 NUTRITION & DIETETICS Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI:10.3164/jcbn.22-107
Tomohisa Takagi, Tadao Kunihiro, Shunsuke Takahashi, Takayoshi Hisada, Koji Nagashima, Jun Mochizuki, Katsura Mizushima, Yuji Naito
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Abstract

Recent studies have revealed that the gut microbiome affects various health conditions via its metabolites, including short-chain fatty acids (SCFAs) and bile acids (BAs). In the analysis of these, appropriate collection, handling, and storage of fecal specimens are required, and convenient specimen handling processes will facilitate their investigation. Here, we developed a novel preservation solution, "Metabolokeeper®", to stabilize fecal microbiota, organic acids including SCFAs, and BAs at room temperature. In the present study, we collected fecal samples from 20 healthy adult volunteers and stored them at room temperature with Metabolokeeper® and at -80°C without preservatives for up to four weeks to evaluate the usefulness of the novel preservative solution. We found that microbiome profiles and short chain fatty acid contents were stably maintained at room temperature with Metabolokeeper® for 28 days, while the bile acids were stably maintained for 7 days under the same conditions. We conclude that this convenient procedure to obtain a fecal sample for collecting the gut microbiome and gut metabolites can contribute to a better understanding of the health effects of fecal metabolites produced by the gut microbiome.

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一种用于保存粪便标本中的短链脂肪酸、胆汁酸和微生物群的新开发的溶液。
最近的研究表明,肠道微生物组通过其代谢物,包括短链脂肪酸(SCFAs)和胆汁酸(BAs),影响各种健康状况。在这些分析中,需要适当的收集、处理和储存粪便标本,方便的标本处理过程将有助于对其进行调查。在这里,我们开发了一种新的保存溶液“Metabolokeeper®”,用于在室温下稳定粪便微生物群、有机酸(包括scfa)和ba。在本研究中,我们收集了20名健康成人志愿者的粪便样本,并使用metabolkeeper®在室温下和-80°C不含防腐剂的条件下保存了4周,以评估这种新型防腐剂溶液的有效性。我们发现,在相同条件下,使用metabolkeeper®可在室温下稳定维持28天的微生物群特征和短链脂肪酸含量,而胆汁酸含量可稳定维持7天。我们的结论是,这种获取粪便样本以收集肠道微生物组和肠道代谢物的便捷程序有助于更好地了解肠道微生物组产生的粪便代谢物对健康的影响。
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来源期刊
CiteScore
4.30
自引率
8.30%
发文量
57
审稿时长
6-12 weeks
期刊介绍: Journal of Clinical Biochemistry and Nutrition (JCBN) is an international, interdisciplinary publication encompassing chemical, biochemical, physiological, pathological, toxicological and medical approaches to research on lipid peroxidation, free radicals, oxidative stress and nutrition. The Journal welcomes original contributions dealing with all aspects of clinical biochemistry and clinical nutrition including both in vitro and in vivo studies.
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