The effect of encomir-93 mimic transfection on the expression of miR-93 and PSA and androgen receptor in prostate cancer LNcap cell line.

IF 1.1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Hormone Molecular Biology and Clinical Investigation Pub Date : 2023-03-31 eCollection Date: 2023-09-01 DOI:10.1515/hmbci-2022-0096

Hossein Mokhtari, Ali Ebrahimi, Mohaddeseh Nejati, Zeinab Barartabar, Mehdi Damchi, Abbas Khonakdar-Tarsi, Mahlagha Zahedi

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Abstract

Objectives: Prostate cancer (PCa) is one of the most common cancers in men with high mortality rate which is a major concern for men's health. However, the molecular mechanisms remain poorly understood. miR-93 is an important oncogene which may have important function in prostate cancer.So, this study aimed to predict that encomir-93 mimic transfection on the expression of miR-93 and PSA and AR in prostate cancer LNcap cell line.

Methods: Lymph node carcinoma of the prostate (LNCaP) was cultured and then miR-93 mimics was designed, synthesized and the transfected to LNCaP. The expression level of prostate-specific antigen (PSA) and androgen receptor (AR) was determined via Real-time PCR after treated with 15 pmol of miR-93 mimics.

Results: miR-93 mimic transfection led to significant increase in PSA and AR expression in comparison with control group (p≤0.05).

Conclusions: The miR-93 and its target genes has important role in PCa progression via enhancement in PSA and AR expression. Further research on the function of the miR-93 and its target genes in tumorgenesis and progression PCa could be helpful for the treatment of prostate cancer.

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encomir-93模拟转染对前列腺癌症LNcap细胞系中miR-93、PSA和雄激素受体表达的影响。

目的：癌症（PCa）是男性最常见的癌症之一，死亡率高，是男性健康的主要问题。然而，对其分子机制仍知之甚少。miR-93是一种重要的癌基因，可能在癌症中发挥重要作用。因此，本研究旨在预测encomir-93模拟转染对前列腺癌症LNcap细胞系中miR-93、PSA和AR表达的影响。方法：培养前列腺淋巴结癌（LNCaP），设计、合成miR-93模拟物并转染LNCaP。用15pmol的miR-93模拟物处理后，通过实时PCR测定前列腺特异性抗原（PSA）和雄激素受体（AR）的表达水平。结果：与对照组相比，miR-93模拟转染导致PSA和AR表达显著增加（p≤0.05）。结论：miR-93及其靶基因通过增强PSA和AR的表达，在前列腺癌的进展中发挥重要作用。进一步研究miR-93及其靶基因在肿瘤发生和进展PCa中的作用可能有助于癌症的治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hormone Molecular Biology and Clinical Investigation BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.60

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0.00%

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期刊介绍： Hormone Molecular Biology and Clinical Investigation (HMBCI) is dedicated to the provision of basic data on molecular aspects of hormones in physiology and pathophysiology. The journal covers the treatment of major diseases, such as endocrine cancers (breast, prostate, endometrium, ovary), renal and lymphoid carcinoma, hypertension, cardiovascular systems, osteoporosis, hormone deficiency in menopause and andropause, obesity, diabetes, brain and related diseases, metabolic syndrome, sexual dysfunction, fetal and pregnancy diseases, as well as the treatment of dysfunctions and deficiencies. HMBCI covers new data on the different steps and factors involved in the mechanism of hormone action. It will equally examine the relation of hormones with the immune system and its environment, as well as new developments in hormone measurements. HMBCI is a blind peer reviewed journal and publishes in English: Original articles, Reviews, Mini Reviews, Short Communications, Case Reports, Letters to the Editor and Opinion papers. Ahead-of-print publishing ensures faster processing of fully proof-read, DOI-citable articles.