Imbalance of Microbacterial Diversity Is Associated with Functional Prognosis of Stroke.

IF 3 4区 医学 Q2 NEUROSCIENCES Neural Plasticity Pub Date : 2023-01-01 DOI:10.1155/2023/6297653
Xintong Zhang, Xiangyu Wang, Hong Zhao, Risheng Cao, Yini Dang, Binbin Yu
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引用次数: 2

Abstract

Objectives: There is mounting evidence to suggest that the pathophysiology of stroke is greatly influenced by the microbiota of the gut and its metabolites, in particular short-chain fatty acids (SCFAs). The primary purpose of the study was to evaluate whether the levels of SCFAs and the gut microbiota are altered in poststroke patients and to examine the relationship between these alterations and the physical condition, intestinal health, pain, or nutritional status of patients.

Methods: Twenty stroke patients and twenty healthy controls were enrolled in the current study, and their demographics were matched. Gas chromatography was used to determine the fecal SCFAs, and 16S rRNA gene sequencing was used to evaluate their fecal microbiota. Microbial diversity and richness were examined using the diversity indices alpha and beta, and taxonomic analysis was utilized to determine group differences. The relationships between the gut microbiome and fecal SCFAs, discriminant bacteria, and poststroke clinical outcomes were analyzed.

Results: Less community richness (ACE and Chao) was observed in the poststroke patients (P < 0.05), but the differences between the poststroke group and the healthy control group in terms of species diversity (Shannon and Simpson) were not statistically significant. The makeup of the poststroke gut microbiota was distinct from that of the control group, as evidenced by beta diversity. Then, the relative abundances of the taxa in the poststroke and control groups were compared in order to identify the specific microbiota changes. At the level of phylum, the poststroke subjects showed a significant increase in the relative abundances of Akkermansiaceae, Fusobacteriota, Desulfobacterota, Ruminococcaceae, and Oscillospirales and a particularly noticeable decrease in the relative abundance of Acidobacteriota compared to the control subjects (P < 0.05). In regard to SCFA concentrations, lower levels of fecal acetic acid (P = 0.001) and propionic acid (P = 0.049) were found in poststroke subjects. Agathobacter was highly correlated with acetic acid level (r = 0.473, P = 0.002), whereas Fusobacteria (r = -0.371, P = 0.018), Flavonifractor (r = -0.334, P = 0.034), Desulfovibrio (r = -0.362, P = 0.018), and Akkermansia (r = -0.321, P = 0.043) were negatively related to acetic acid levels. Additionally, the findings of the correlation analysis revealed that Akkermansia (r = -0.356, P = 0.024), Desulfovibrio (r = -0.316, P = 0.047), and Alloprevotella (r = -0.366, P = 0.020) were significantly negatively correlated with high-density lipoprotein cholesterol. In addition, the Neurogenic Bowel Dysfunction score (r = 0.495, P = 0.026), Barthel index (r = -0.531, P = 0.015), Fugl-Meyer Assessment score (r = -0.565, P = 0.009), Visual Analogue Scale score (r = 0.605, P = 0.005), and Brief Pain Inventory score (r = 0.507, P = 0.023) were significantly associated with alterations of distinctive gut microbiota.

Conclusions: Stroke generates extensive and substantial alterations in the gut microbiota and SCFAs, according to our findings. The differences of intestinal flora and lower fecal SCFA levels are closely related to the physical function, intestinal function, pain, or nutritional status of poststroke patients. Treatment strategies aimed at modulating the gut microbiota and SCFAs may have the potential to enhance the clinical results of patients.

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微生物多样性失衡与脑卒中功能预后相关。
目的:越来越多的证据表明,中风的病理生理在很大程度上受肠道微生物群及其代谢物,特别是短链脂肪酸(SCFAs)的影响。该研究的主要目的是评估中风后患者的SCFAs水平和肠道微生物群是否发生改变,并检查这些改变与患者的身体状况、肠道健康、疼痛或营养状况之间的关系。方法:将20例脑卒中患者和20例健康对照纳入本研究,并进行人口统计学匹配。采用气相色谱法测定粪便SCFAs,采用16S rRNA基因测序法评价其粪便微生物群。利用多样性指数alpha和beta测定微生物多样性和丰富度,并利用分类学分析确定类群差异。分析肠道微生物组与粪便SCFAs、鉴别细菌和脑卒中后临床结局之间的关系。结果:脑卒中后患者群落丰富度(ACE和Chao)低于正常对照组(P < 0.05),但物种多样性(Shannon和Simpson)与正常对照组差异无统计学意义。中风后肠道微生物群的组成与对照组不同,贝塔多样性证明了这一点。然后,比较脑卒中后和对照组的相对丰度,以确定具体的微生物群变化。在门水平上,卒中后受试者Akkermansiaceae、Fusobacteriota、Desulfobacterota、Ruminococcaceae和Oscillospirales的相对丰度显著高于对照组,而Acidobacteriota的相对丰度显著低于对照组(P < 0.05)。在SCFA浓度方面,卒中后受试者的粪便乙酸(P = 0.001)和丙酸(P = 0.049)水平较低。无水杆菌与乙酸水平呈高度相关(r = 0.473, P = 0.002),梭杆菌(r = -0.371, P = 0.018)、黄酮因子(r = -0.334, P = 0.034)、脱硫弧菌(r = -0.362, P = 0.018)、Akkermansia (r = -0.321, P = 0.043)与乙酸水平呈负相关(r = -0.321, P = 0.043)。相关性分析结果显示,Akkermansia (r = -0.356, P = 0.024)、Desulfovibrio (r = -0.316, P = 0.047)、Alloprevotella (r = -0.366, P = 0.020)与高密度脂蛋白胆固醇呈显著负相关。此外,神经源性肠功能障碍评分(r = 0.495, P = 0.026)、Barthel指数(r = -0.531, P = 0.015)、Fugl-Meyer评估评分(r = -0.565, P = 0.009)、视觉模拟量表评分(r = 0.605, P = 0.005)和短暂疼痛量表评分(r = 0.507, P = 0.023)与不同肠道菌群的改变显著相关。结论:根据我们的研究结果,中风在肠道微生物群和scfa中产生广泛而实质性的改变。脑卒中后患者肠道菌群差异及粪便SCFA水平降低与机体功能、肠道功能、疼痛或营养状况密切相关。旨在调节肠道微生物群和scfa的治疗策略可能有可能提高患者的临床结果。
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来源期刊
Neural Plasticity
Neural Plasticity NEUROSCIENCES-
CiteScore
6.80
自引率
0.00%
发文量
77
审稿时长
16 weeks
期刊介绍: Neural Plasticity is an international, interdisciplinary journal dedicated to the publication of articles related to all aspects of neural plasticity, with special emphasis on its functional significance as reflected in behavior and in psychopathology. Neural Plasticity publishes research and review articles from the entire range of relevant disciplines, including basic neuroscience, behavioral neuroscience, cognitive neuroscience, biological psychology, and biological psychiatry.
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