Effects of melatonin on hematologic and biochemical changes and the effects of circadian rhythm on hematologic changes in donkeys (Equus asinus) subjected to packing during the hot-dry season

IF 1.1 4区农林科学 Q3 VETERINARY SCIENCES Veterinary clinical pathology Pub Date : 2023-02-13 DOI:10.1111/vcp.13193

Ayodele Stephen Ake, Joseph Olusegun Ayo, Tagang Aluwong, Aliyu Mohammed

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引用次数: 1

Abstract

Background

Melatonin is a hormone with antioxidant and anti-inflammatory properties and may alleviate the effects of stress on hematologic and biochemical analytes in working donkeys that pack (load-carry).

Objectives

We aimed to evaluate the effects of melatonin administration on hematologic and biochemical responses in donkeys subjected to packing during the hot-dry season. We also examined its effects on circadian rhythms.

Methods

Ten male Nubian pack donkeys with (Packing + Melatonin; P + M) and without melatonin (Packing-Melatonin; P-M) administration were included. Blood samples were collected before, immediately after (15 min), and from 16 h to over 27-h postpacking at 3-h intervals for hematologic and total protein analyses. Serum from blood samples collected before and immediately after packing was analyzed for muscle enzyme activities and electrolyte concentrations. Student's t-test, one-way ANOVA, and online cosinor analysis were used for statistical analyses.

Results

Packed cell volumes (PCVs) of the P + M donkeys did not differ before and after packing. However, for the P-M donkeys, values before packing (30.20 ± 0.8%) were higher than those (26.87 ± 0.5%) after packing. The hematologic values recorded in the P + M donkeys were higher than those in the P-M donkeys, especially during the scotophase (P ˂ 0.05). The mesor PCV, hemoglobin (Hb), and red blood cell (RBC) values for the P + M donkeys were significantly (P ˂ 0.05) higher than those in the P-M donkeys. Lactate dehydrogenase (LDH) activity was not different before (289.40 ± 60.8 U/L) or after packing (323.20 ± 36.0 U/L) in P + M donkeys, but the activity was significantly (P ˂ 0.05) higher postpacking than prepacking in the P-M donkeys (294.20 ± 66.9 U/L and 513.40 ± 68.5 U/L, respectively). The relationships between the meteorologic parameters and PCV, Hb, and RBC concentrations in the P + M donkeys were significantly negative (P ˂ 0.05).

Conclusions

Melatonin prevented a decrease in PCV and increase in LDH activity immediately after packing compared with non-treated donkeys. There was a marked effect during the scotophase on PCV, Hb, and RBC concentrations. The circadian parameters indicated a significant effect of melatonin on the rhythmicity of the PCV, Hb, and RBC concentration in the treated donkeys. Melatonin administration may reduce the risk of adverse effects of environmental stress and stress due to packing in donkeys during the hot-dry season.

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褪黑素对干热季节包装驴血液生化变化的影响及昼夜节律对血液变化的影响

褪黑素是一种具有抗氧化和抗炎特性的激素，可以缓解应激对驮运驴血液和生化分析的影响。目的研究褪黑素对干热季节包装驴血液生化反应的影响。我们还研究了它对昼夜节律的影响。方法10头努比亚公驴用(Packing +褪黑素)包装;P + M)，不含褪黑素(packaging -褪黑素;P-M)给药。在包装前、包装后立即(15分钟)和包装后16小时至27小时以上采集血样，每隔3小时进行血液学和总蛋白分析。对包装前和包装后立即采集的血清进行肌肉酶活性和电解质浓度分析。采用学生t检验、单因素方差分析和在线余弦分析进行统计分析。结果P + M毛驴包装前后的包装细胞体积(PCVs)无显著差异。而P-M毛驴在包装前(30.20±0.8%)高于包装后(26.87±0.5%)。P + M组的血液学指标高于P-M组，特别是在黄斑期(P小于0.05)。P + M组的内膜PCV、血红蛋白(Hb)和红细胞(RBC)值显著高于P-M组(P小于0.05)。P + M组乳酸脱氢酶(LDH)活性在包装前(289.40±60.8 U/L)和包装后(323.20±36.0 U/L)差异不显著，但包装后(294.20±66.9 U/L)和包装前(513.40±68.5 U/L)显著高于P + M组(P小于0.05)。气象参数与P + M毛驴体内PCV、Hb、RBC浓度呈显著负相关(P小于0.05)。结论:与未处理的驴相比，褪黑素在包装后立即阻止PCV的下降和LDH活性的增加。斑疹期对PCV、Hb和RBC浓度有显著影响。昼夜节律参数表明，褪黑素对治疗驴体内PCV、Hb和RBC浓度的节律性有显著影响。褪黑素的服用可以减少环境压力和压力的不利影响的风险，因为驴在炎热干燥的季节装箱。

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来源期刊

Veterinary clinical pathology 农林科学-兽医学

CiteScore

1.70

自引率

16.70%

发文量

133

审稿时长

18-36 weeks

期刊介绍： Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.