Characterizing Lung Particulates Using Quantitative Microscopy in Coal Miners With Severe Pneumoconiosis.

IF 3.7 3区 医学 Q2 MEDICAL LABORATORY TECHNOLOGY Archives of pathology & laboratory medicine Pub Date : 2024-03-01 DOI:10.5858/arpa.2022-0427-OA
Jeremy T Hua, Carlyne D Cool, Heather A Lowers, Leonard H T Go, Lauren M Zell-Baran, Emily A Sarver, Kirsten S Almberg, Kathy D Pang, Susan M Majka, Angela D Franko, Naseema I Vorajee, Robert A Cohen, Cecile S Rose
{"title":"Characterizing Lung Particulates Using Quantitative Microscopy in Coal Miners With Severe Pneumoconiosis.","authors":"Jeremy T Hua, Carlyne D Cool, Heather A Lowers, Leonard H T Go, Lauren M Zell-Baran, Emily A Sarver, Kirsten S Almberg, Kathy D Pang, Susan M Majka, Angela D Franko, Naseema I Vorajee, Robert A Cohen, Cecile S Rose","doi":"10.5858/arpa.2022-0427-OA","DOIUrl":null,"url":null,"abstract":"<p><strong>Context.—: </strong>Current approaches for characterizing retained lung dust using pathologists' qualitative assessment or scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS) have limitations.</p><p><strong>Objective.—: </strong>To explore polarized light microscopy coupled with image-processing software, termed quantitative microscopy-particulate matter (QM-PM), as a tool to characterize in situ dust in lung tissue of US coal miners with progressive massive fibrosis.</p><p><strong>Design.—: </strong>We developed a standardized protocol using microscopy images to characterize the in situ burden of birefringent crystalline silica/silicate particles (mineral density) and carbonaceous particles (pigment fraction). Mineral density and pigment fraction were compared with pathologists' qualitative assessments and SEM/EDS analyses. Particle features were compared between historical (born before 1930) and contemporary coal miners, who likely had different exposures following changes in mining technology.</p><p><strong>Results.—: </strong>Lung tissue samples from 85 coal miners (62 historical and 23 contemporary) and 10 healthy controls were analyzed using QM-PM. Mineral density and pigment fraction measurements with QM-PM were comparable to consensus pathologists' scoring and SEM/EDS analyses. Contemporary miners had greater mineral density than historical miners (186 456 versus 63 727/mm3; P = .02) and controls (4542/mm3), consistent with higher amounts of silica/silicate dust. Contemporary and historical miners had similar particle sizes (median area, 1.00 versus 1.14 μm2; P = .46) and birefringence under polarized light (median grayscale brightness: 80.9 versus 87.6; P = .29).</p><p><strong>Conclusions.—: </strong>QM-PM reliably characterizes in situ silica/silicate and carbonaceous particles in a reproducible, automated, accessible, and time/cost/labor-efficient manner, and shows promise as a tool for understanding occupational lung pathology and targeting exposure controls.</p>","PeriodicalId":8305,"journal":{"name":"Archives of pathology & laboratory medicine","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of pathology & laboratory medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5858/arpa.2022-0427-OA","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Context.—: Current approaches for characterizing retained lung dust using pathologists' qualitative assessment or scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS) have limitations.

Objective.—: To explore polarized light microscopy coupled with image-processing software, termed quantitative microscopy-particulate matter (QM-PM), as a tool to characterize in situ dust in lung tissue of US coal miners with progressive massive fibrosis.

Design.—: We developed a standardized protocol using microscopy images to characterize the in situ burden of birefringent crystalline silica/silicate particles (mineral density) and carbonaceous particles (pigment fraction). Mineral density and pigment fraction were compared with pathologists' qualitative assessments and SEM/EDS analyses. Particle features were compared between historical (born before 1930) and contemporary coal miners, who likely had different exposures following changes in mining technology.

Results.—: Lung tissue samples from 85 coal miners (62 historical and 23 contemporary) and 10 healthy controls were analyzed using QM-PM. Mineral density and pigment fraction measurements with QM-PM were comparable to consensus pathologists' scoring and SEM/EDS analyses. Contemporary miners had greater mineral density than historical miners (186 456 versus 63 727/mm3; P = .02) and controls (4542/mm3), consistent with higher amounts of silica/silicate dust. Contemporary and historical miners had similar particle sizes (median area, 1.00 versus 1.14 μm2; P = .46) and birefringence under polarized light (median grayscale brightness: 80.9 versus 87.6; P = .29).

Conclusions.—: QM-PM reliably characterizes in situ silica/silicate and carbonaceous particles in a reproducible, automated, accessible, and time/cost/labor-efficient manner, and shows promise as a tool for understanding occupational lung pathology and targeting exposure controls.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
用定量显微镜对严重肺炎矿工肺部颗粒物特征的研究。
上下文。--:目前使用病理学家的定性评估或使用能量色散光谱(SEM/EDS)的扫描电子显微镜来表征残留的肺尘的方法具有局限性。目标。--:探索偏振光显微镜与图像处理软件(称为定量显微镜颗粒物(QM-PM))相结合,作为表征美国煤矿工人进行性大面积纤维化肺组织中原位灰尘的工具。设计。--:我们开发了一个标准化方案,使用显微镜图像来表征双折射晶体二氧化硅/硅酸盐颗粒(矿物密度)和碳质颗粒(颜料部分)的原位负载。将矿物密度和色素组分与病理学家的定性评估和SEM/EDS分析进行比较。对历史上(1930年之前出生)和当代煤矿工人的颗粒特征进行了比较,他们在采矿技术变化后可能有不同的暴露。结果。--:使用QM-PM分析来自85名煤矿工人(62名历史矿工和23名当代矿工)和10名健康对照的肺组织样本。QM-PM的矿物密度和色素组分测量结果与公认病理学家的评分和SEM/EDS分析结果相当。当代矿工的矿物密度高于历史矿工(186 456对63 727/mm3;P=0.02)和对照组(4542/mm3),与较高量的二氧化硅/硅酸盐粉尘一致。当代和历史上的矿工具有相似的粒度(中值面积,1.00对1.14μm2;P=.46)和偏振光下的双折射(中值灰度亮度:80.9对87.6;P=.29),并显示出作为了解职业肺部病理学和靶向暴露控制的工具的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
9.20
自引率
2.20%
发文量
369
审稿时长
3-8 weeks
期刊介绍: Welcome to the website of the Archives of Pathology & Laboratory Medicine (APLM). This monthly, peer-reviewed journal of the College of American Pathologists offers global reach and highest measured readership among pathology journals. Published since 1926, ARCHIVES was voted in 2009 the only pathology journal among the top 100 most influential journals of the past 100 years by the BioMedical and Life Sciences Division of the Special Libraries Association. Online access to the full-text and PDF files of APLM articles is free.
期刊最新文献
New Entities and Concepts in Salivary Gland Tumor Pathology: The Role of Molecular Alterations. Update on Sinonasal Tract Malignancies: Advances in Diagnostic Modalities. Update on Salivary Gland Fine-Needle Aspiration and the Milan System for Reporting Salivary Gland Cytopathology. BRAF Exon 15 Mutations in the Evaluation of Well-Differentiated Epithelial Nephroblastic Neoplasms in Children: A Report From the Children's Oncology Group Study AREN03B2. Neoplastic Progression in Intraductal Papillary Neoplasm of the Bile Duct.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1