Single-cell transcriptome profiling reveals distinct expression patterns among genes in the mouse incisor dental pulp.

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY International Journal of Developmental Biology Pub Date : 2023-01-01 DOI:10.1387/ijdb.220173db

Badam Enkhmandakh, Dashzeveg Bayarsaihan

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引用次数: 0

Abstract

SOX transcription factors play key roles in cell differentiation and cell fate determination during development. Using single-cell RNA-sequencing data, we examined the expression profiles of Sox genes in the mouse incisor dental pulp. Our analysis showed that Sox4, Sox5, Sox9, Sox11, and Sox12 are mainly expressed in mesenchymal stem/stromal cells (MSCs) representing osteogenic cells at different stages of differentiation. We found that in several MSCs, Sox genes co-expressed with regulatory genes such as Sp7, Satb2, Msx1, Snai2, Dlx1, Twist2, and Tfap2a. In addition, Sox family genes colocalized with Runx2 and Lef1, which are highly enriched in MSCs undergoing osteoblast differentiation. A protein interaction network analysis uncovered that CREBBP, CEBPB, TLE1, TWIST1, and members of the HDAC and SMAD families are interacting partners of RUNX2 and LEF1 during skeletal development. Collectively, the distinct expression patterns of the SOX transcription factors suggest that they play essential regulatory roles in directing lineage-specific gene expression during differentiation of MSCs.

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单细胞转录组分析揭示了小鼠门牙牙髓中不同基因的表达模式。

SOX转录因子在细胞分化和发育过程中决定细胞命运中起着关键作用。利用单细胞rna测序数据，我们检测了Sox基因在小鼠切牙牙髓中的表达谱。我们的分析表明，Sox4、Sox5、Sox9、Sox11和Sox12主要在代表成骨细胞不同分化阶段的间充质干细胞/基质细胞(MSCs)中表达。我们发现在一些间质干细胞中，Sox基因与调控基因如Sp7、Satb2、Msx1、Snai2、Dlx1、Twist2和Tfap2a共表达。此外，Sox家族基因与Runx2和Lef1共定位，在成骨细胞分化的MSCs中高度富集。一项蛋白质相互作用网络分析发现，CREBBP、CEBPB、TLE1、TWIST1以及HDAC和SMAD家族成员在骨骼发育过程中是RUNX2和LEF1的相互作用伙伴。总的来说，SOX转录因子的不同表达模式表明它们在MSCs分化过程中指导谱系特异性基因表达方面发挥着重要的调节作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Developmental Biology 生物-发育生物学

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： The International Journal of Developmental Biology (ISSN: 0214- 6282) is an independent, not for profit scholarly journal, published by scientists, for scientists. The journal publishes papers which throw light on our understanding of animal and plant developmental mechanisms in health and disease and, in particular, research which elucidates the developmental principles underlying stem cell properties and cancer. Technical, historical or theoretical approaches also fall within the scope of the journal. Criteria for acceptance include scientific excellence, novelty and quality of presentation of data and illustrations. Advantages of publishing in the journal include: rapid publication; free unlimited color reproduction; no page charges; free publication of online supplementary material; free publication of audio files (MP3 type); one-to-one personalized attention at all stages during the editorial process. An easy online submission facility and an open online access option, by means of which papers can be published without any access restrictions. In keeping with its mission, the journal offers free online subscriptions to academic institutions in developing countries.