MiR-199a-3p promotes repair of myocardial infarction by targeting NACC2.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-01-01

Xue-Zheng Wang, Lei Chen, Hao Sun, Xiao-Qian Li, Hu Wang, Xiao-Peng Zhang, Jiang-Bin Sun, Hai-Yong Wang

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引用次数: 0

Abstract

Objective: Myocardial infarction (MI) has gained widespread interest due to its high death and disability rate worldwide. Some miRNAs are markers of heart disease. Therefore, it is necessary to understand the mechanism for repairing MI injury.

Methods: Here, we evaluated the relative expression levels of miR-199a-3p in mouse and human myocardial cell models of injury, and its effect on myocardial cells viability using Cell Counting Kit-8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridline (EdU) assay, and flow cytometry assay as well as western blot in vitro. Furthermore, we performed bioinformatic online analysis to investigate the role that miR-199a-3p plays in cardiomyocyte injury, measured by dual-luciferase reporter assay.

Results: The results showed that miR-199a-3p significantly increased the growth rate of cardiomyocytes after treating them with hydrogen peroxide (H₂O₂). miR-199a-3p also acted as an inhibitor that directly targeted NACC2, resulting in a higher NACC2 expression level in the injury model of cardiomyocytes than normal myocardial cells and thus preventing miR-199a-3p-induced proliferation promotion in model cardiomyocytes.

Conclusion: Our results demonstrate that miR-199a-3p may be a prognostic biomarker in myocardial injury.

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MiR-199a-3p通过靶向NACC2促进心肌梗死的修复。

目的:心肌梗死(MI)因其高致死率和致残率在世界范围内引起了广泛关注。一些mirna是心脏病的标志。因此，了解心肌梗死损伤的修复机制是十分必要的。方法:采用细胞计数试剂盒-8 (CCK-8)法、5-乙基-2′-脱氧尿苷(EdU)法、体外流式细胞术及western blot检测miR-199a-3p在小鼠和人损伤心肌细胞模型中的相对表达水平及其对心肌细胞活力的影响。此外，我们进行了生物信息学在线分析，以研究miR-199a-3p在心肌细胞损伤中的作用，通过双荧光素酶报告基因测定。结果:结果显示，过氧化氢(H2O2)处理心肌细胞后，miR-199a-3p显著提高心肌细胞的生长速度。miR-199a-3p还作为直接靶向NACC2的抑制剂，导致心肌细胞损伤模型中NACC2的表达水平高于正常心肌细胞，从而阻止了miR-199a-3p诱导的模型心肌细胞增殖促进。结论:我们的研究结果表明miR-199a-3p可能是心肌损伤的预后生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.