Association of mesenchymal stem cells derived from bone marrow and adipose tissue enhances bone repair in rat calvarial defects.

IF 2.4 4区医学 Q4 CELL & TISSUE ENGINEERING Regenerative medicine Pub Date : 2023-05-01 DOI:10.2217/rme-2022-0219

Gabriela Guaraldo Campos Totoli, Rayana Longo Bighetti-Trevisan, Gileade Pereira Freitas, Leticia Faustino Adolpho, Adriana Luisa Golçalves Almeida, Ana Carolina Loyola Barbosa, Jaqueline Isadora Reis Ramos, Marcio Mateus Beloti, Adalberto Luiz Rosa

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引用次数: 2

Abstract

Aim: We evaluated the bone repair induced by MSCs from adipose tissue (AT-MSCs) and bone marrow (BM-MSCs) injected into rat calvarial defects at two time points. Methods & results: Both cell populations expressed MSC surface markers and differentiated into adipocytes and osteoblasts. μCT showed that the combination of cells from distinct sources exhibited synergistic effects to increase bone repair with an advantage when BM-MSCs were injected prior to AT-MSCs. The higher osteogenic potential of these MSC combinations was demonstrated using an in vitro coculture system where BM-MSCs and AT-MSCs association induced higher ALP activity in MC3T3-E1 cells. Conclusion: Our findings may drive new approaches to treat bone defects and shed light on the complexity of the mechanisms involved in bone regeneration.

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骨髓间充质干细胞与脂肪组织的结合促进了大鼠颅骨缺损的骨修复。

目的:观察脂肪组织间充质干细胞(at -MSCs)和骨髓间充质干细胞(BM-MSCs)在两个时间点对大鼠颅骨缺损的骨修复作用。方法与结果:两组细胞均表达MSC表面标记，并分化为脂肪细胞和成骨细胞。μCT结果显示，不同来源的细胞组合在促进骨修复方面表现出协同效应，且在AT-MSCs之前注射BM-MSCs具有优势。在体外共培养系统中，BM-MSCs和AT-MSCs联合诱导MC3T3-E1细胞中更高的ALP活性，证明了这些MSC组合具有更高的成骨潜能。结论:我们的发现可能会推动治疗骨缺损的新方法，并揭示骨再生机制的复杂性。

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来源期刊

Regenerative medicine 医学-工程：生物医学

CiteScore

4.20

自引率

3.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization. Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community. Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.

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