Streptococcus mutans dexA affects exopolysaccharides production and biofilm homeostasis.

IF 2.8 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Molecular Oral Microbiology Pub Date : 2023-04-01 DOI:10.1111/omi.12395

Yang Yan, He Hailun, Yang Fenghui, Liu Pingting, Lei Lei, Zhao Zhili, Hu Tao

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引用次数: 1

Abstract

Objectives: The study aimed to evaluate the role of Streptococcus mutans (S. mutans) dexA gene on biofilm structure and microecological distribution in multispecies biofilms.

Materials and methods: A multispecies biofilm model consisting of S. mutans and its dexA mutants, Streptococcus gordonii (S. gordonii) and Streptococcus sanguinis (S. sanguinis) was constructed, and bacterial growth, biofilm architecture and microbiota composition were determined to study the effect of the S. mutans dexA on multispecies biofilms.

Results: Our results showed that either deletion or overexpression of S. mutans dexA had no effect on the planktonic growth of bacterium, while S. mutans dominated in the multispecies biofilms to form cariogenic biofilms. Furthermore, we revealed that the SmudexA+ group showed structural abnormality in the form of more fractures and blank areas. The morphology of the SmudexA group was sparser and more porous, with reduced and less agglomerated exopolysaccharides scaffold. Interestingly, the microbiota composition analysis provided new insights that the inhibition of S. gordonii and S. sanguinis was alleviated in the SmudexA group compared to the significantly suppressed condition in the other groups.

Conclusion: In conclusion, deletion of S. mutans dexA gene re-modules biofilm structure and microbiota composition, thereby leading to decreased cariogenicity. Thus, the S. mutans dexA may be an important target for regulating the cariogenicity of dental plaque biofilms, expecting to be a probiotic for caries control.

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变形链球菌dexA影响胞外多糖的产生和生物膜的稳态。

目的:研究变形链球菌dexA基因对多物种生物膜结构及微生态分布的影响。材料与方法:构建由变形链球菌及其dexA突变体、戈登链球菌(S. gordonii)和血链球菌(S. sanguinis)组成的多物种生物膜模型，测定细菌生长、生物膜结构和微生物群组成，研究变形链球菌dexA对多物种生物膜的影响。结果:我们的研究结果表明，突变链球菌dexA的缺失或过表达对细菌的浮游生长没有影响，而突变链球菌在多物种生物膜中占主导地位，形成致龋生物膜。此外，我们发现SmudexA+组表现出结构异常，表现为更多的骨折和空白区域。SmudexA组细胞形态更稀疏，多孔性更强，胞外多糖支架减少，结块少。有趣的是，微生物群组成分析提供了新的见解，与其他组的显著抑制情况相比，SmudexA组减轻了对gordonii和S. sanguinis的抑制。结论:总之，突变链球菌dexA基因的缺失重组了生物膜结构和菌群组成，从而降低了致癌性。因此，变形链球菌dexA可能是调控牙菌斑生物膜致龋性的重要靶点，有望成为防治龋齿的益生菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY

CiteScore

6.50

自引率

5.40%

发文量

审稿时长

>12 weeks

期刊介绍： Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.