Single-cell A/B testing for cell-cell communication.

IF 9 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Cell Systems Pub Date : 2023-06-21 DOI:10.1016/j.cels.2023.05.006

Caitlin M Aamodt, Nathan E Lewis

引用次数: 0

Abstract

A new method developed by Francisco Quintana's group, systematic perturbation of encapsulated associated cells followed by sequencing (SPEAC-seq), applies a CRISPR screen to co-cultured interacting cells to identify the ligands mediating cell-cell communication. Using this approach, the authors discover the molecular basis of a microglia-astrocyte feedback loop that suppresses neuroinflammatory disease.

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用于蜂窝通信的单蜂窝 A/B 测试。

弗朗西斯科-金塔纳（Francisco Quintana）研究小组开发的一种新方法--系统扰动包囊关联细胞并测序（SPEAC-seq）--将CRISPR筛选应用于共培养的相互作用细胞，以确定介导细胞间通讯的配体。利用这种方法，作者发现了抑制神经炎性疾病的小胶质细胞-胃细胞反馈环路的分子基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Systems Medicine-Pathology and Forensic Medicine

CiteScore

16.50

自引率

1.10%

发文量

审稿时长

42 days

期刊介绍： In 2015, Cell Systems was founded as a platform within Cell Press to showcase innovative research in systems biology. Our primary goal is to investigate complex biological phenomena that cannot be simply explained by basic mathematical principles. While the physical sciences have long successfully tackled such challenges, we have discovered that our most impactful publications often employ quantitative, inference-based methodologies borrowed from the fields of physics, engineering, mathematics, and computer science. We are committed to providing a home for elegant research that addresses fundamental questions in systems biology.