Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes.

IF 2.5 4区 医学 Q3 GENETICS & HEREDITY Mutagenesis Pub Date : 2023-06-20 DOI:10.1093/mutage/gead013
Andrea Pirković, Vesna Lazić, Biljana Spremo-Potparević, Lada Živković, Dijana Topalović, Sanja Kuzman, Jelena Antić-Stanković, Dragana Božić, Milica Jovanović Krivokuća, Jovan M Nedeljković
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Abstract

Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20-25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025-0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.

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橄榄叶提取物和橄榄苦苷功能化Ag NPs对人滋养细胞和外周血淋巴细胞毒性的比较分析。
以干橄榄叶提取物(DOLE)及其活性成分橄榄苦苷(OLE)为还原剂和稳定剂,制备了20-25 nm的胶体银纳米颗粒(Ag NPs)。利用透射电子显微镜、x射线衍射分析和吸收光谱对银纳米粒子进行了表征。研究了包被Ag NPs及其无机和有机成分对滋养细胞、人外周血淋巴细胞(pbl)、革兰氏阳性、革兰氏阴性细菌和酵母的细胞毒性作用。用彗星试验评估pbl体外遗传毒性。当银浓度为0.025 ~ 0.2 mM时,Ag/DOLE和Ag/OLE均可诱导两种细胞在暴露24 h后产生细胞毒作用,但Ag/OLE的细胞毒作用最为明显。在0.1 mM和0.2 mM的情况下,这两种胶体也会导致两种细胞类型的ROS产生减少,而在任何条件下,裸Ag NPs都不会改变ROS水平。功能化Ag/DOLE和Ag/OLE在PBLs中没有表现出基因毒性作用,而裸AgNPs仅在0.2 mM处显著增加DNA损伤。抑菌作用方面,Ag/OLE在0.0625 ~ 0.0312 mM范围内具有MIC值,且Ag/DOLE对革兰氏阴性菌和酵母的抑菌作用显著高于革兰氏阳性菌。获得的结果表明,Ag/OLE诱导了最显著的生物效应,有利于其作为抗菌剂的应用,但暴露于高浓度可能导致健康人体细胞毒性的潜在风险。
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来源期刊
Mutagenesis
Mutagenesis 生物-毒理学
CiteScore
5.90
自引率
3.70%
发文量
22
审稿时长
6-12 weeks
期刊介绍: Mutagenesis is an international multi-disciplinary journal designed to bring together research aimed at the identification, characterization and elucidation of the mechanisms of action of physical, chemical and biological agents capable of producing genetic change in living organisms and the study of the consequences of such changes.
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