High expression of GPR50 promotes the proliferation, migration and autophagy of hepatocellular carcinoma cells in vitro.

IF 3.6 3区生物学 Q3 CELL BIOLOGY Journal of Cell Communication and Signaling Pub Date : 2023-12-01 Epub Date: 2023-06-28 DOI:10.1007/s12079-023-00772-9

Weiming Zhao, Lingling Xi, Guoying Yu, Gaiping Wang, Cuifang Chang

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Abstract

G protein-coupled receptors (GPCRs) play important roles in tumorigenesis and the development of hepatocellular carcinoma (HCC). GPR50 is an orphan GPCR. Previous studies have indicated that GPR50 could protect against breast cancer development and decrease tumor growth in a xenograft mouse model. However, its role in HCC remains indistinct. To detect the role and the regulation mechanism of GPR50 in HCC, GPR50 expression was analyzed in HCC patients (gene expression omnibus database (GEO) (GSE45436)) and detected in HCC cell line CBRH-7919, and the results showed that GPR50 was significantly up-regulated in HCC patients and CBRH-7919 cell line compared to the corresponding normal control. Gpr50 cDNA was transfected into HCC cell line CBRH-7919, and we found that Gpr50 promoted the proliferation, migration, and autophagy of CBRH-7919. The regulation mechanism of GPR50 in HCC was detected by isobaric tags for relative and absolute quantification (iTRAQ) analysis, and we found that GPR50 promoted HCC was closely related to CCT6A and PGK1. Taken together, GPR50 may promote HCC progression via CCT6A-induced proliferation and PGK1-induced migration and autophagy, and GPR50 could be an important target for HCC.

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高表达 GPR50 可促进体外肝癌细胞的增殖、迁移和自噬。

G 蛋白偶联受体（GPCR）在肿瘤发生和肝细胞癌（HCC）发展过程中发挥着重要作用。GPR50 是一种孤儿 GPCR。以前的研究表明，GPR50 可以防止乳腺癌的发展，并在异种移植小鼠模型中减少肿瘤的生长。然而，它在 HCC 中的作用仍不明确。为了检测GPR50在HCC中的作用和调控机制，研究人员分析了GPR50在HCC患者中的表达（基因表达总括数据库（GEO）（GSE45436）），并检测了HCC细胞株CBRH-7919中的表达，结果表明与相应的正常对照相比，GPR50在HCC患者和CBRH-7919细胞株中显著上调。将Gpr50 cDNA转染到HCC细胞株CBRH-7919中，我们发现Gpr50能促进CBRH-7919的增殖、迁移和自噬。通过同位素标签相对和绝对定量（iTRAQ）分析检测了GPR50在HCC中的调控机制，发现GPR50对HCC的促进作用与CCT6A和PGK1密切相关。综上所述，GPR50可能通过CCT6A诱导的增殖和PGK1诱导的迁移和自噬促进HCC的进展，GPR50可能是HCC的一个重要靶点。

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来源期刊

Journal of Cell Communication and Signaling CELL BIOLOGY-

CiteScore

6.40

自引率

4.90%

发文量

期刊介绍： The Journal of Cell Communication and Signaling provides a forum for fundamental and translational research. In particular, it publishes papers discussing intercellular and intracellular signaling pathways that are particularly important to understand how cells interact with each other and with the surrounding environment, and how cellular behavior contributes to pathological states. JCCS encourages the submission of research manuscripts, timely reviews and short commentaries discussing recent publications, key developments and controversies. Research manuscripts can be published under two different sections : In the Pathology and Translational Research Section (Section Editor Andrew Leask) , manuscripts report original research dealing with celllular aspects of normal and pathological signaling and communication, with a particular interest in translational research. In the Molecular Signaling Section (Section Editor Satoshi Kubota) manuscripts report original signaling research performed at molecular levels with a particular interest in the functions of intracellular and membrane components involved in cell signaling.